Lgl1 activation of Rab10 Promotes axonal membrane trafficking underlying neuronal polarization

Wang, Tong, Liu, Yang, Xu, Xiao-Hui, Deng, Cai-Yun, Wu, Kong-Yan, Zhu, Ji, Fu, Xiu-Qing, He, Miao and Luo, Zhen-Ge (2011) Lgl1 activation of Rab10 Promotes axonal membrane trafficking underlying neuronal polarization. Developmental Cell, 21 3: 431-444. doi:10.1016/j.devcel.2011.07.007


Author Wang, Tong
Liu, Yang
Xu, Xiao-Hui
Deng, Cai-Yun
Wu, Kong-Yan
Zhu, Ji
Fu, Xiu-Qing
He, Miao
Luo, Zhen-Ge
Title Lgl1 activation of Rab10 Promotes axonal membrane trafficking underlying neuronal polarization
Journal name Developmental Cell   Check publisher's open access policy
ISSN 1534-5807
1878-1551
Publication date 2011-09-13
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.devcel.2011.07.007
Open Access Status
Volume 21
Issue 3
Start page 431
End page 444
Total pages 14
Place of publication Cambridge, MA United States
Publisher Cell Press
Collection year 2011
Language eng
Abstract Directed membrane trafficking is believed to be crucial for axon development during neuronal morphogenesis. However, the underlying mechanisms are poorly understood. Here, we report a role of Lgl1, the mammalian homolog of Drosophila tumor suppressor Lethal giant larvae, in controlling membrane trafficking underlying axonal growth. We find that Lgl1 is associated with plasmalemmal precursor vesicles and enriched in developing axons. Lgl1 upregulation promoted axonal growth, whereas downregulation attenuated it as well as directional membrane insertion. Interestingly, Lgl1 interacted with and activated Rab10, a small GTPase that mediates membrane protein trafficking, by releasing GDP dissociation inhibitor (GDI) from Rab10. Furthermore, Rab10 lies downstream of Lgl1 in axon development and directional membrane insertion. Finally, both Lgl1 and Rab10 are required for neocortical neuronal polarization in vivo. Thus, the Lgl1 regulation of Rab10 stimulates the trafficking of membrane precursor vesicles, whose fusion with the plasmalemma is crucial for axonal growth.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Fri, 24 Oct 2014, 16:32:00 EST by Sylvie Pichelin on behalf of Queensland Brain Institute