Helical cyclic pentapeptides constrain HIV-1 Rev peptide for enhanced RNA binding

Harrison, Rosemary S., Shepherd, Nicholas E., Hoang, Huy N., Beyer, Renee L., Ruiz-Gomez, Gloria, Kelso, Michael J., Mei Kok, W., Hill, Timothy A., Abbenante, Giovanni and Fairlie, David P. (2014) Helical cyclic pentapeptides constrain HIV-1 Rev peptide for enhanced RNA binding. Tetrahedron, 70 42: 7645-7650. doi:10.1016/j.tet.2014.07.096

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Author Harrison, Rosemary S.
Shepherd, Nicholas E.
Hoang, Huy N.
Beyer, Renee L.
Ruiz-Gomez, Gloria
Kelso, Michael J.
Mei Kok, W.
Hill, Timothy A.
Abbenante, Giovanni
Fairlie, David P.
Title Helical cyclic pentapeptides constrain HIV-1 Rev peptide for enhanced RNA binding
Journal name Tetrahedron   Check publisher's open access policy
ISSN 1464-5416
0040-4020
Publication date 2014-10-21
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.tet.2014.07.096
Volume 70
Issue 42
Start page 7645
End page 7650
Total pages 6
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2015
Language eng
Abstract HIV-1 Rev is a 116 residue transporter protein that enters the host cell nucleus and uses its 17 amino acid segment (Rev34–50) to bind and capture a specific piece of RNA, the Rev Response Element (RRE), for transport to the cytoplasm. This is critical for HIV replication. In isolation, Rev34–50 shows negligible structure in water, but is alpha helical in a mixture of water and 2,2,2-trifluoroethanol (TFE) or when bound to RRE. Here we report that helix-constrained cyclic pentapeptides, either appended to the N-terminus or incorporated within Rev34–50, are efficient helix nucleators in water. They induce up to 90% alpha helicity for isolated Rev peptides in water and confer high RNA-binding affinity.
Keyword Cyclic peptide
Alpha helix
HIV-1 Rev
RNA
Helix induction
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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