Equine lamellar energy metabolism studied using tissue microdialysis

Medina-Torres, C. E., Pollitt, C. C., Underwood, C., Castro-Olivera, E. M., Collins, S. N., Allavena, R. E., Richardson, D. W. and van Eps, A. W. (2014) Equine lamellar energy metabolism studied using tissue microdialysis. Veterinary Journal, 201 3: 275-282. doi:10.1016/j.tvjl.2014.05.030

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Author Medina-Torres, C. E.
Pollitt, C. C.
Underwood, C.
Castro-Olivera, E. M.
Collins, S. N.
Allavena, R. E.
Richardson, D. W.
van Eps, A. W.
Title Equine lamellar energy metabolism studied using tissue microdialysis
Journal name Veterinary Journal   Check publisher's open access policy
ISSN 1090-0233
Publication date 2014-09
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.tvjl.2014.05.030
Open Access Status
Volume 201
Issue 3
Start page 275
End page 282
Total pages 8
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2015
Language eng
Subject 3400 Veterinary
1103 Clinical Sciences
Formatted abstract
Failure of lamellar energy metabolism may contribute to the pathophysiology of equine laminitis. Tissue microdialysis has the potential to dynamically monitor lamellar energy balance over time. The objectives of this study were to develop a minimally invasive lamellar microdialysis technique and use it to measure normal lamellar energy metabolite concentrations over 24 h. Microdialysis probes were placed (through the white line) into either the lamellar dermis (LAM) (n = 6) or the sublamellar dermis (SUBLAM) (n = 6) and perfused continuously over a 24 h study period. Probes were placed in the skin dermis (SKIN) for simultaneous comparison to LAM (n = 6). Samples were collected every 2 h and analysed for glucose, lactate, pyruvate, urea and glycerol concentrations. LAM was further compared with SUBLAM by simultaneous placement and sampling in four feet from two horses over 4 h. Horses were monitored for lameness, and either clinically evaluated for 1 month after probe removal (n = 4) or subjected to histological evaluation of the probe site (n = 10).

There were no deleterious clinical effects of probe placement and the histological response was mild. Sample fluid recovery and metabolite concentrations were stable for 24 h. Glucose was lower (and lactate:glucose ratio higher) in LAM compared with SUBLAM and SKIN (P < 0.05). Pyruvate was lower in SUBLAM than SKIN and urea was lower in LAM than SKIN (P < 0.05). These differences suggest lower perfusion and increased glucose consumption in LAM compared with SUBLAM and SKIN. In conclusion, lamellar tissue microdialysis was well tolerated and may be useful for determining the contribution of energy failure in laminitis pathogenesis.
Keyword Bioenergetics
Urea clearance
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Veterinary Science Publications
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