Distinct miRNA signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke Opisthorchis viverrini

Plieskatt, Jordan L., Rinaldi, Gabriel, Feng, Yanjun, Peng, Jin, Yonglitthipagon, Ponlapat, Easley, Samantha, Laha, Therawach, Pairojkul, Chawalit, Bhudhisawasdi, Vajarabhongsa, Sripa, Banchob, Brindley, Paul J., Mulvenna, Jason P. and Bethony, Jeffrey M. (2014) Distinct miRNA signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke Opisthorchis viverrini. Journal of Hepatology, 61 4: 850-858. doi:10.1016/j.jhep.2014.05.035

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Author Plieskatt, Jordan L.
Rinaldi, Gabriel
Feng, Yanjun
Peng, Jin
Yonglitthipagon, Ponlapat
Easley, Samantha
Laha, Therawach
Pairojkul, Chawalit
Bhudhisawasdi, Vajarabhongsa
Sripa, Banchob
Brindley, Paul J.
Mulvenna, Jason P.
Bethony, Jeffrey M.
Title Distinct miRNA signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke Opisthorchis viverrini
Formatted title
Distinct miRNA signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke Opisthorchis viverrini
Journal name Journal of Hepatology   Check publisher's open access policy
ISSN 0168-8278
1600-0641
Publication date 2014-10
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.jhep.2014.05.035
Open Access Status
Volume 61
Issue 4
Start page 850
End page 858
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
Background & Aims: Intrahepatic cholangiocarcinoma (ICC) is a significant public health problem in East Asia, where it is strongly associated with chronic infection by the food-borne parasite Opisthorchis viverrini (OV). We report the first comprehensive miRNA expression profiling by microarray of the most common histologic grades and subtypes of ICC: well differentiated, moderately differentiated, and papillary ICC.

Methods: MicroRNA expression profiles from FFPE were compared among the following: ICC tumour tissue (n = 16), non-tumour tissue distally macrodissected from the same ICC tumour block (n = 15), and normal tissue (n = 13) from individuals undergoing gastric bypass surgery. A panel of deregulated miRNAs was validated by qPCR.

Results: Each histologic grade and subtype of ICC displayed a distinct miRNA profile, with no cohort of miRNAs emerging as commonly deregulated. Moderately differentiated ICC showed the greatest miRNA deregulation in quantity and magnitude, followed by the papillary subtype, and then well differentiated ICC. Moreover, when ICC tumour tissues were compared to adjacent non-tumour tissue, similar miRNA dysregulation profiles were observed.

Conclusions: We show that common histologic grades and subtypes of ICC have distinct miRNA profiles. As histological grade and subtypes are associated with ICC aggressiveness, these profiles could be used to enhance the early detection and improve the personalised treatment for ICC. These findings also suggest the involvement of specific miRNAs during ICC tumour progression and differentiation. We plan to use these insights to (a) detect these profiles in circulation and (b) conduct functional analyses to decipher the roles of miRNAs in ICC tumour differentiation.
Keyword MicroRNA
Cholangiocarcinoma
Intrahepatic cholangiocarcinoma
Histological grade
Opisthorchis viverrini
Microarray
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biological Sciences Publications
 
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