Incorporating target sequences of developmentally regulated small RNAs into transgenes to enhance tissue specificity of expression in plants

Moyle, Richard L., Sternes, Peter R. and Birch, Robert G. (2014) Incorporating target sequences of developmentally regulated small RNAs into transgenes to enhance tissue specificity of expression in plants. Plant Molecular Biology Reporter, 33 3: 505-511. doi:10.1007/s11105-014-0765-6


Author Moyle, Richard L.
Sternes, Peter R.
Birch, Robert G.
Title Incorporating target sequences of developmentally regulated small RNAs into transgenes to enhance tissue specificity of expression in plants
Journal name Plant Molecular Biology Reporter   Check publisher's open access policy
ISSN 0735-9640
1572-9818
Publication date 2014-08-01
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s11105-014-0765-6
Open Access Status
Volume 33
Issue 3
Start page 505
End page 511
Total pages 7
Place of publication New York NY United States
Publisher Springer New York
Collection year 2015
Language eng
Abstract Many applications of plant genetic engineering require tight control of transgene expression to a particular organ, tissue or developmental stage, particularly when off-target expression causes deleterious effects. Most studies have relied on tissue-specific and developmentally regulated promoters to confer the desired transgene expression pattern via transcriptional control. However, the process to identify promoters conferring required expression patterns is slow and expensive, with no certainty of success. Therefore, we investigated the practicality of post-transcriptional control of transgene expression patterns in plants, triggered by binding of endogenous small RNA sequences. Target sequences of several small RNAs, inserted 3′ of a luciferase reporter, altered transgene expression in patterns consistent with developmental abundance of the corresponding small RNAs in sugarcane. Incorporating the target sequences of miR167 or a leaf-specific small RNA selectively reduced reporter expression in leaves by more than 90 % on average, whereas incorporating the miR160-3p target sequence selectively reduced reporter expression in roots by 75 % on average across immature transgenic lines. We anticipate that the strategy should be broadly applicable in plants.
Keyword Post-transcriptional control
miRNA
Saccharum
miR167
miR160
Transgene expression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahead of print 01 Aug 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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