Development and characterization of a novel, antimicrobial, sterile hydrogel dressing for burn wounds: Single-step production with gamma irradiation creates silver nanoparticles and radical polymerization

Boonkaew, B., Barber, P.M., Rengpipat, S., Supaphol, P., Kempf, M., He, J., John, V.T. and Cuttle, L. (2014) Development and characterization of a novel, antimicrobial, sterile hydrogel dressing for burn wounds: Single-step production with gamma irradiation creates silver nanoparticles and radical polymerization. Journal of Pharmaceutical Sciences, 103 10: 3244-3253. doi:10.1002/jps.24095


Author Boonkaew, B.
Barber, P.M.
Rengpipat, S.
Supaphol, P.
Kempf, M.
He, J.
John, V.T.
Cuttle, L.
Title Development and characterization of a novel, antimicrobial, sterile hydrogel dressing for burn wounds: Single-step production with gamma irradiation creates silver nanoparticles and radical polymerization
Journal name Journal of Pharmaceutical Sciences   Check publisher's open access policy
ISSN 0022-3549
1520-6017
Publication date 2014
Sub-type Article (original research)
DOI 10.1002/jps.24095
Open Access Status
Volume 103
Issue 10
Start page 3244
End page 3253
Total pages 10
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Collection year 2015
Language eng
Subject 3003 Pharmaceutical Science
Abstract Patients with burn wounds are susceptible to wound infection and sepsis. This research introduces a novel burn wound dressing that contains silver nanoparticles (SNPs) to treat infection in a 2-acrylamido-2-methylpropane sulfonic acid sodium salt (AMPS-Na+) hydrogel. Silver nitrate was dissolved in AMPS-Na+ solution and then exposed to gamma irradiation to form SNP-infused hydrogels. The gamma irradiation results in a cross-linked polymeric network of sterile hydrogel dressing and a reduction of silver ions to form SNPs infused in the hydrogel in a one-step process. About 80% of the total silver was released from the hydrogels after 72 h immersion in simulated body fluid solution; therefore, they could be used on wounds for up to 3 days. All the hydrogels were found to be nontoxic to normal human dermal fibroblast cells. The silver-loaded hydrogels had good inhibitory action against Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus. Results from a pilot study on a porcine burn model showed that the 5-mM silver hydrogel was efficient at preventing bacterial colonization of wounds, and the results were comparable to the commercially available silver dressings (ActicoatTM, PolyMem Silver®). These results support its use as a potential burn wound dressing.
Keyword Antimicrobial activity
Burn wounds
Cell culture
Dressings
Gamma irradiation
Hydrogels
Nanoparticles
Polymer synthesis
Silver
Spectroscopy
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Queensland Children's Medical Research Institute Publications
School of Medicine Publications
 
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