Total transcriptome, proteome, and allergome of Johnson grass pollen, which is important for allergic rhinitis in subtropical regions

Campbell, Bradley C., Gilding, Edward K., Timbrell, Victoria, Guru, Preethi, Loo, Dorothy, Zennaro, Danila, Mari, Adriano, Solley, Graham, Hill, Michelle M., Godwin, Ian D. and Davies, Janet M. (2015) Total transcriptome, proteome, and allergome of Johnson grass pollen, which is important for allergic rhinitis in subtropical regions. Journal of Allergy and Clinical Immunology, 135 1: 133-142. doi:10.1016/j.jaci.2014.06.034


Author Campbell, Bradley C.
Gilding, Edward K.
Timbrell, Victoria
Guru, Preethi
Loo, Dorothy
Zennaro, Danila
Mari, Adriano
Solley, Graham
Hill, Michelle M.
Godwin, Ian D.
Davies, Janet M.
Title Total transcriptome, proteome, and allergome of Johnson grass pollen, which is important for allergic rhinitis in subtropical regions
Journal name Journal of Allergy and Clinical Immunology   Check publisher's open access policy
ISSN 0091-6749
1097-6825
Publication date 2015-01
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.jaci.2014.06.034
Open Access Status
Volume 135
Issue 1
Start page 133
End page 142
Total pages 10
Place of publication Philadelphia, United States
Publisher Mosby
Collection year 2015
Language eng
Formatted abstract
Background

Genomic data are lacking for many allergen sources. To circumvent this limitation, we implemented a strategy to reveal the repertoire of pollen allergens of a grass with clinical importance in subtropical regions, where an increasing proportion of the world's population resides.

Objective

We sought to identify and immunologically characterize the allergenic components of the Panicoideae Johnson grass pollen (JGP; Sorghum halepense).

Methods

The total pollen transcriptome, proteome, and allergome of JGP were documented. Serum IgE reactivities with pollen and purified allergens were assessed in 64 patients with grass pollen allergy from a subtropical region.

Results

Purified Sor h 1 and Sor h 13 were identified as clinically important allergen components of JGP with serum IgE reactivity in 49 (76%) and 28 (43.8%), respectively, of patients with grass pollen allergy. Within whole JGP, multiple cDNA transcripts and peptide spectra belonging to grass pollen allergen families 1, 2, 4, 7, 11, 12, 13, and 25 were identified. Pollen allergens restricted to subtropical grasses (groups 22-24) were also present within the JGP transcriptome and proteome. Mass spectrometry confirmed the IgE-reactive components of JGP included isoforms of Sor h 1, Sor h 2, Sor h 13, and Sor h 23.

Conclusion

Our integrated molecular approach revealed qualitative differences between the allergenic components of JGP and temperate grass pollens. Knowledge of these newly identified allergens has the potential to improve specific diagnosis and allergen immunotherapy treatment for patients with grass pollen allergy in subtropical regions and reduce the burden of allergic respiratory disease globally.
Keyword Allergome
Allergen components
Allergic rhinitis
IgE
Johnson grass pollen
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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