Parenteral administration of GnRH constructs and adjuvants: immune responses and effects on reproductive tissues of male mice

Sharma, Sameer, McDonald, Ian, Miller, Lowell and Hinds, Lyn A. (2014) Parenteral administration of GnRH constructs and adjuvants: immune responses and effects on reproductive tissues of male mice. Vaccine, 32 43: 5555-5563. doi:10.1016/j.vaccine.2014.07.075


Author Sharma, Sameer
McDonald, Ian
Miller, Lowell
Hinds, Lyn A.
Title Parenteral administration of GnRH constructs and adjuvants: immune responses and effects on reproductive tissues of male mice
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-410X
1873-2518
Publication date 2014-09-29
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2014.07.075
Open Access Status
Volume 32
Issue 43
Start page 5555
End page 5563
Total pages 9
Place of publication London United Kingdom
Publisher Elsevier
Collection year 2015
Language eng
Abstract Two gonadotrophin releasing hormone (GnRH) constructs prepared by either chemical conjugation to keyhole limpet hemocyanin (GnRH-KLH) or as an expressed recombinant fusion protein (Multimer) were evaluated with or without adjuvants (immunostimulating complexes, ISCOMs, or cytosine-phosphate-guanosine oligodeoxynucleotides, CpG ODNs). After subcutaneous administration to Balb/c male mice at Weeks 0, 2 and 4, these preparations were assessed for induction of immune responses and effects on reproductive organs. GnRH-KLH plus ISCOMs formulation induced strong IgG immune responses from Week 4 through Week 12 resulting in consistent reproductive organ atrophy by Week 12 after subcutaneous administration. GnRH-KLH plus CpG ODNs generated immune responses but no atrophy of reproductive tissues by Week 12. Multimer plus ISCOMs induced poor immune responses and no effects on reproductive tissues by Week 12. In the absence of additional adjuvant, none of the GnRH constructs induced reproductive organ atrophy. GnRH-KLH induced stronger immune responses when formulated with ISCOMs or CpG ODN compared to Multimer. GnRH-KLH with ISCOMs could be an effective colloidal alternative for emulsion GnRH vaccine formulations.
Keyword Vaccine
Endogenous peptide
Immunostimulating complexes
Cytosine-phosphate-guanosine oligodeoxynucleotides
Testis
Epididymis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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