Marker genes identify three somatic cell types in the fetal mouse ovary

Rastetter, Raphael H., Bernard, Pascal, Palmer, James S., Chassot, Anne-Amandine, Chen, Huijun, Western, Patrick S., Ramsay, Robert G., Chaboissier, Marie-Christine and Wilhelm, Dagmar (2014) Marker genes identify three somatic cell types in the fetal mouse ovary. Developmental Biology, 394 2: 242-252. doi:10.1016/j.ydbio.2014.08.013

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Author Rastetter, Raphael H.
Bernard, Pascal
Palmer, James S.
Chassot, Anne-Amandine
Chen, Huijun
Western, Patrick S.
Ramsay, Robert G.
Chaboissier, Marie-Christine
Wilhelm, Dagmar
Title Marker genes identify three somatic cell types in the fetal mouse ovary
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
Publication date 2014
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2014.08.013
Open Access Status File (Author Post-print)
Volume 394
Issue 2
Start page 242
End page 252
Total pages 11
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2015
Abstract The two main functions of the ovary are the production of oocytes, which allows the continuation of the species, and secretion of female sex hormones, which control many aspects of female development and physiology. Normal development of the ovaries during embryogenesis is critical for their function and the health of the individual in later life. Although the adult ovary has been investigated in great detail, we are only starting to understand the cellular and molecular biology of early ovarian development. Here we show that the adult stem cell marker Lgr5 is expressed in the cortical region of the fetal ovary and this expression is mutually exclusive to FOXL2. Strikingly, a third somatic cell population can be identified, marked by the expression of NR2F2, which is expressed in LGR5- and FOXL2 double-negative ovarian somatic cells. Together, these three marker genes label distinct ovarian somatic cell types. Using lineage tracing in mice, we show that Lgr5-positive cells give rise to adult cortical granulosa cells, which form the follicles of the definitive reserve. Moreover, LGR5 is required for correct timing of germ cell differentiation as evidenced by a delay of entry into meiosis in Lgr5 loss-of-function mutants, demonstrating a key role for LGR5 in the differentiation of pre-granulosa cells, which ensure the differentiation of oogonia, the formation of the definitive follicle reserve, and long-term female fertility.
Keyword Foxl2
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
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