In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds

Rodero, Mathieu P., Licata, Fabrice, Poupel, Lucie, Hamon, Pauline, Khosrotehrani, Kiarash, Combadiere, Christophe and Boissonnas, Alexandre (2014) In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds. PLoS One, 9 10: e108212.1-e108212.9. doi:10.1371/journal.pone.0108212


Author Rodero, Mathieu P.
Licata, Fabrice
Poupel, Lucie
Hamon, Pauline
Khosrotehrani, Kiarash
Combadiere, Christophe
Boissonnas, Alexandre
Title In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-10-01
Sub-type Article (original research)
DOI 10.1371/journal.pone.0108212
Open Access Status DOI
Volume 9
Issue 10
Start page e108212.1
End page e108212.9
Total pages 9
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
The cells of the mononuclear phagocyte system are essential for the correct healing of adult skin wounds, but their specific functions remain ill-defined. The absence of granulation tissue immediately after skin injury makes it challenging to study the role of mononuclear phagocytes at the initiation of this inflammatory stage. To study their recruitment and migratory behavior within the wound bed, we developed a new model for real-time in vivo imaging of the wound, using transgenic mice that express green and cyan fluorescent proteins and specifically target monocytes. Within hours after the scalp injury, monocytes invaded the wound bed. The complete abrogation of this infiltration in monocyte-deficient CCR2-/- mice argues for the involvement of classical monocytes in this process. Monocyte infiltration unexpectedly occurred as early as neutrophil recruitment did and resulted from active release from the bloodstream toward the matrix through microhemorrhages rather than transendothelial migration. Monocytes randomly scouted around the wound bed, progressively slowed down, and stopped. Our approach identified and characterized a rapid and earlier than expected wave of monocyte infiltration and provides a novel framework for investigating the role of these cells during early stages of wound healing.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Tue, 21 Oct 2014, 00:23:54 EST by System User on behalf of UQ Centre for Clinical Research