Effect of atrazine and fenitrothion at no-observed-effect-levels (NOEL) on amphibian and mammalian corticosterone-binding-globulin (CBG)

Hernandez, Sandra E., Sernia, Conrad and Bradley, Adrian J. (2014) Effect of atrazine and fenitrothion at no-observed-effect-levels (NOEL) on amphibian and mammalian corticosterone-binding-globulin (CBG). Toxicology Letters, 230 3: 408-412. doi:10.1016/j.toxlet.2014.08.015


Author Hernandez, Sandra E.
Sernia, Conrad
Bradley, Adrian J.
Title Effect of atrazine and fenitrothion at no-observed-effect-levels (NOEL) on amphibian and mammalian corticosterone-binding-globulin (CBG)
Journal name Toxicology Letters   Check publisher's open access policy
ISSN 0378-4274
1879-3169
Publication date 2014-11-04
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.toxlet.2014.08.015
Volume 230
Issue 3
Start page 408
End page 412
Total pages 5
Place of publication Shannon, Co. Clare, Ireland
Publisher Elsevier Ireland
Collection year 2015
Language eng
Abstract This study determines the effect of atrazine and fenitrothion no-observed-effect-levels (NOEL) on the binding of corticosterone (B) to corticosterone-binding-globulin (CBG) in an amphibian and a mammal. Plasma from five cane toads and five Wistar rats was exposed to atrazine and fenitrothion at the NOEL approved for Australian fresh water residues and by the World Health Organization (WHO). The concentration required to displace 50% (IC50) of B binding to CBG was determined by a competitive microdialysis protein assay. Competition studies showed that both atrazine and fenitrothion at NOEL are able to compete with B for CBG binding sites in toad and rat plasma. The IC50 levels for atrazine in toads and rats were 0.004 nmol/l and 0.09 nmol/l respectively. In the case of fenitrothion the IC50 level found in toads was 0.007 nmol/l, and 0.025 nmol/l in rats. Plasma dilution curves showed parallelism with the curve of B, demonstrating that these agro-chemicals are competitively inhibiting binding to CBG. The displacement of B by atrazine and fenitrothion would affect the total:free ratio of B and consequently disrupt the normal stress response. This is the first time that the potential disruptive effect of atrazine and fenitrothion on B-CBG interaction at the NOELs has been demonstrated in amphibian and mammalian models.
Keyword NOEL
Atrazine
Fenitrothion
CBG
Amphibian
Rat
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
 
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