Primary cilia function regulates the length of the embryonic trunk axis and urogenital field in mice

Wainwright, Elanor N., Svingen, Terje, Ng, Ee Ting, Wicking, Carol and Koopman, Peter (2014) Primary cilia function regulates the length of the embryonic trunk axis and urogenital field in mice. Developmental Biology, 395 2: 342-354. doi:10.1016/j.ydbio.2014.08.037


Author Wainwright, Elanor N.
Svingen, Terje
Ng, Ee Ting
Wicking, Carol
Koopman, Peter
Title Primary cilia function regulates the length of the embryonic trunk axis and urogenital field in mice
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
1095-564X
Publication date 2014-11-15
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2014.08.037
Volume 395
Issue 2
Start page 342
End page 354
Total pages 13
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2015
Language eng
Formatted abstract
The issues of whether and how some organs coordinate their size and shape with the blueprint of the embryo axis, while others appear to regulate their morphogenesis autonomously, remain poorly understood. Mutations in Ift144, encoding a component of the trafficking machinery of primary cilia assembly, result in a range of embryo patterning defects, affecting the limbs, skeleton and neural system. Here, we show that embryos of the mouse mutant Ift144twt develop gonads that are larger than wild-type. Investigation of the early patterning of the urogenital ridge revealed that the anterior–posterior domain of the gonad/mesonephros was extended at 10.5 dpc, with no change in the length of the metanephros. In XY embryos, this extension resulted in an increase in testis cord number. Moreover, we observed a concomitant extension of the trunk axis in both sexes, with no change in the length of the tail domain or somite number. Our findings support a model in which: (1) primary cilia regulate embryonic trunk elongation; (2) the length of the trunk axis determines the size of the urogenital ridges; and (3) the gonad domain is partitioned into a number of testis cords that depends on the available space, rather than being divided a predetermined number of times to generate a specific number of cords.
Keyword Gonad
Testis
Ovary
Organogenesis
Embryo patterning
Primary cilia
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 16 Oct 2014, 00:17:46 EST by Susan Allen on behalf of Institute for Molecular Bioscience