Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis

Turvey, Michelle E., Koudelka, Tomas, Comerford, Iain, Greer, Judith M., Carroll, William, Bernard, Claude C. A., Hoffmann, Peter and McColl, Shaun R. (2014) Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis. Journal of Proteome Research, 13 8: 3655-3670. doi:10.1021/pr500158r


Author Turvey, Michelle E.
Koudelka, Tomas
Comerford, Iain
Greer, Judith M.
Carroll, William
Bernard, Claude C. A.
Hoffmann, Peter
McColl, Shaun R.
Title Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis
Formatted title
Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis
Journal name Journal of Proteome Research   Check publisher's open access policy
ISSN 1535-3893
1535-3907
Publication date 2014-08-01
Sub-type Article (original research)
DOI 10.1021/pr500158r
Open Access Status
Volume 13
Issue 8
Start page 3655
End page 3670
Total pages 16
Place of publication Washington, DC, United States
Publisher American Chemical Society
Collection year 2015
Language eng
Formatted abstract
Experimental autoimmune encephalomyelitis (EAE) is a murine model of multiple sclerosis, a chronic neurodegenerative and inflammatory autoimmune condition of the central nervous system (CNS). Pathology is driven by the infiltration of autoreactive CD4+ lymphocytes into the CNS, where they attack neuronal sheaths causing ascending paralysis. We used an isotope-coded protein labeling approach to investigate the proteome of CD4+ cells isolated from the spinal cord and brain of mice at various stages of EAE progression in two EAE disease models: PLP139–151-induced relapsing-remitting EAE and MOG35–55-induced chronic EAE, which emulate the two forms of human multiple sclerosis. A total of 1120 proteins were quantified across disease onset, peak-disease, and remission phases of disease, and of these 13 up-regulated proteins of interest were identified with functions relating to the regulation of inflammation, leukocyte adhesion and migration, tissue repair, and the regulation of transcription/translation. Proteins implicated in processes such as inflammation (S100A4 and S100A9) and tissue repair (annexin A1), which represent key events during EAE progression, were validated by quantitative PCR. This is the first targeted analysis of autoreactive cells purified from the CNS during EAE, highlighting fundamental CD4+ cell-driven processes that occur during the initiation of relapse and remission stages of disease.
Keyword Autoimmunity
Experimental autoimmune encephalomyelitis (EAE)
CD4+ T lymphocyte
Isotope-coded protein labeling (ICPL)
MALDI-TOF/TOF
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2015 Collection
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 24 Sep 2014, 14:48:39 EST by Judith M Greer on behalf of UQ Centre for Clinical Research