Control of chitin and N-acetylglucosamine utilization in Saccharopolyspora erythraea

Liao, Chengheng, Rigali, Sebastien, Cassani, Cuauhtemoc Licona, Marcellin, Esteban, Nielsen, Lars Keld and Ye, Bang-Ce (2014) Control of chitin and N-acetylglucosamine utilization in Saccharopolyspora erythraea. Microbiology (United Kingdom), 160 9: 1914-1928. doi:10.1099/mic.0.078261-0

Author Liao, Chengheng
Rigali, Sebastien
Cassani, Cuauhtemoc Licona
Marcellin, Esteban
Nielsen, Lars Keld
Ye, Bang-Ce
Title Control of chitin and N-acetylglucosamine utilization in Saccharopolyspora erythraea
Formatted title
Control of chitin and N-acetylglucosamine utilization in Saccharopolyspora erythraea
Journal name Microbiology (United Kingdom)   Check publisher's open access policy
ISSN 1465-2080
Publication date 2014-09
Year available 2014
Sub-type Article (original research)
DOI 10.1099/mic.0.078261-0
Open Access Status
Volume 160
Issue 9
Start page 1914
End page 1928
Total pages 15
Place of publication Reading, Berks United Kingdom
Publisher Society for General Microbiology
Collection year 2015
Language eng
Formatted abstract
Chitin degradation and subsequent N-acetylglucosamine (GlcNAc) catabolism is thought to be a common trait of a large majority of actinomycetes. Utilization of aminosugars had been poorly investigated outside the model strain Streptomyces coelicolor A3(2), and we examined here the genetic setting of the erythromycin producer Saccharopolyspora erythraea for GlcNAc and chitin utilization, as well as the transcriptional control thereof. Sacch. erythraea efficiently utilize GlcNAc most likely via the phosphotransferase system (PTSGlcNAc); however, this strain is not able to grow when chitin or N,N′-diacetylchitobiose [(GlcNAc)2] is the sole nutrient source, despite a predicted extensive chitinolytic system (chi genes). The inability of Sacch. erythraea to utilize chitin and (GlcNAc)2 is probably because of the loss of genes encoding the DasABC transporter for (GlcNAc)2 import, and genes for intracellular degradation of (GlcNAc)2 by β-N-acetylglucosaminidases. Transcription analyses revealed that in Sacch. erythraea all putative chi and GlcNAc utilization genes are repressed by DasR, whereas in Strep. coelicolor DasR displayed either activating or repressing functions whether it targets genes involved in the polymer degradation or genes for GlcNAc dimer and monomer utilization, respectively. A transcriptomic analysis further showed that GlcNAc not only activates the transcription of GlcNAc catabolism genes but also activates chi gene expression, as opposed to the previously reported GlcNAc-mediated catabolite repression in Strep. coelicolor. Finally, synteny exploration revealed an identical genetic background for chitin utilization in other rare actinomycetes, which suggests that screening procedures that used only the chitin-based protocol for selective isolation of antibiotic-producing actinomycetes could have missed the isolation of many industrially promising strains.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Tue, 23 Sep 2014, 05:02:28 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology