The cellular expression of antiangiogenic factors in fetal primate macula

Kozulin, Peter, Natoli, Riccardo, O'Brien, Keely M. Bumsted, Madigan, Michele C. and Provis, Jan M. (2010) The cellular expression of antiangiogenic factors in fetal primate macula. Investigative Ophthalmology and Visual Science, 51 8: 4298-4306. doi:10.1167/iovs.09-4905

Author Kozulin, Peter
Natoli, Riccardo
O'Brien, Keely M. Bumsted
Madigan, Michele C.
Provis, Jan M.
Title The cellular expression of antiangiogenic factors in fetal primate macula
Journal name Investigative Ophthalmology and Visual Science   Check publisher's open access policy
ISSN 0146-0404
Publication date 2010
Year available 2010
Sub-type Article (original research)
DOI 10.1167/iovs.09-4905
Open Access Status
Volume 51
Issue 8
Start page 4298
End page 4306
Total pages 9
Place of publication Rockville, MD United States
Publisher Association for Research in Vision and Ophthalmology
Collection year 2010
Language eng
Subject 2731 Ophthalmology
2809 Sensory Systems
2804 Cellular and Molecular Neuroscience
Abstract PURPOSE. To characterize the cellular expression patterns of antiangiogenic factorsdifferentially regulated in the fetal human macula. METHODS. RNA was extracted from macular, nasal, and surround biopsies of three human fetal retinas at midgestation. Relative levels of expression of pigment epithelium- derived factor (PEDF), brain natriuretic peptide (BNP), collagen type IV_2 (COL4A2), and natriuretic peptide receptors A and C (NPRA and NPRC) were determined with quantitative PCR. Cellular expression of PEDF and BNP was investigated by in situ hybridization on retinal sections from monkeys aged between fetal day 55 and 11 years. BNP, COL4A2, and NPRA proteins were localized by immunohistochemistry. Labeling was imaged and quantified by confocal microscopy and optical densitometry. RESULTS. Quantitative PCR confirmed higher levels of PEDF and BNP and lower levels of COL4A2 in the macula at midgestation. PEDF mRNA was detected in ganglion cells (GCs) and the pigment epithelium (RPE). BNP mRNA was detected in GCs and macroglia, although BNP immunoreactivity (IR) was predominantly perivascular. COL4A2-IR was detected in large blood vessels and NPRA-IR on the retinal vascular endothelium, GC axons in fetal retinas, and cone axons at all ages. Optical densitometry showed a graded expression of PEDF and BNP at all ages, with highest levels of expression in GCs in the developing fovea. CONCLUSIONS. Because the retinal vessels initially form in the GC layer, it is likely that PEDF has a key role in defining and maintaining the foveal avascular area. The precise role of BNP is unclear, but it may include both antiangiogenic and natriuretic functions.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 16 Sep 2014, 10:14:29 EST by Ms Kate Rowe on behalf of Queensland Brain Institute