eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions

Sina, Abu Ali Ibn, Howell, Sidney, Carrascosa, Laura G., Rauf, Sakandar, Shiddiky, Muhammad J. A . and Trau, Matt (2014) eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions. Chemical Communications, 50 86: 13153-13156. doi:10.1039/C4CC06732K


Author Sina, Abu Ali Ibn
Howell, Sidney
Carrascosa, Laura G.
Rauf, Sakandar
Shiddiky, Muhammad J. A .
Trau, Matt
Title eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions
Journal name Chemical Communications   Check publisher's open access policy
ISSN 1359-7345
1364-548X
Publication date 2014-09-09
Sub-type Article (original research)
DOI 10.1039/C4CC06732K
Open Access Status
Volume 50
Issue 86
Start page 13153
End page 13156
Total pages 4
Place of publication Cambridge, United Kingdom
Publisher R S C Publications
Collection year 2015
Language eng
Abstract We report a simple electrochemical method referred to as “eMethylsorb” for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Thu, 11 Sep 2014, 15:27:39 EST by Dr Muhammad J. A. Shiddiky on behalf of Aust Institute for Bioengineering & Nanotechnology