A structural and functional comparison of nematode and crustacean PDH-like sequences

Meelkop, E., Marco, H. G., Janssen, T., Temmerman, L., Vanhove, M. P. M. and Schoofs, L. (2012) A structural and functional comparison of nematode and crustacean PDH-like sequences. Peptides, 34 1: 74-81. doi:10.3389/fendo.2011.00016


Author Meelkop, E.
Marco, H. G.
Janssen, T.
Temmerman, L.
Vanhove, M. P. M.
Schoofs, L.
Title A structural and functional comparison of nematode and crustacean PDH-like sequences
Journal name Peptides   Check publisher's open access policy
ISSN 0196-9781
1873-5169
Publication date 2012
Sub-type Article (original research)
DOI 10.3389/fendo.2011.00016
Open Access Status
Volume 34
Issue 1
Start page 74
End page 81
Total pages 8
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Abstract The elucidation of the whole genome of the nematode Caenorhabditis elegans allowed for the identification of ortholog genes belonging to the pigment dispersing hormone/factor (PDH/PDF) peptide family. Members of this peptide family are known from crustaceans, insects and nematodes and seem to exist exclusively in ecdysozoans where they play a role in different processes, ranging from the dispersion of integumental and eye (retinal) pigments in decapod crustaceans to circadian rhythms in insects and locomotion in C. elegans. Two pdf genes (pdf-1 and pdf-2) encoding three different peptides: PDF-1a, PDF-1b and PDF-2 have been identified in C. elegans. These three C. elegans PDH-like peptides are similar but not identical in primary structure to PDHs from decapod crustaceans. We investigate whether this divergence has an influence on the pigment dispersing function of the peptides in a decapod crustacean, namely the shrimp Palaemon pacificus. We show that C. elegans PDF-1a and b peptides display cross-functional activity by dispersing pigments in the epithelium of P. pacificus at physiological doses. Moreover, by means of a comparative amino acid sequence analysis of nematode and crustacean PDH-like peptides, we can pinpoint several potentially important residues for eliciting pigment dispersing activity in decapod crustaceans. Although there is no sequence information on a receptor for PDH in decapod crustaceans, we postulate that there is general conservation of the PDH/PDF signaling system based on structural similarities of precursor proteins and receptors (including those from a branchiopod crustacean and from C. elegans).
Keyword Caenorhabditis elegans
PDF receptors
Pigment dispersing factor
Pigment dispersing hormone
Pigment dispersion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Fri, 05 Sep 2014, 11:47:30 EST by Debra McMurtrie on behalf of Queensland Brain Institute