Cytochrome c superstructure biocomposite nucleated by gold nanoparticle: thermal stability and voltammetric behavior

Jiang, Xiue, Shang, Li, Wang, Yuling and Dong, Shaojun (2005) Cytochrome c superstructure biocomposite nucleated by gold nanoparticle: thermal stability and voltammetric behavior. Biomacromolecules, 6 6: 3030-3036. doi:10.1021/bm050345f


Author Jiang, Xiue
Shang, Li
Wang, Yuling
Dong, Shaojun
Title Cytochrome c superstructure biocomposite nucleated by gold nanoparticle: thermal stability and voltammetric behavior
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
1526-4602
Publication date 2005-11-01
Sub-type Article (original research)
DOI 10.1021/bm050345f
Open Access Status Not yet assessed
Volume 6
Issue 6
Start page 3030
End page 3036
Total pages 7
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract The thermal stability of cytochrome c (cyt c) after Au-nanoparticle-directed association has been studied by various spectroscopic (electronic absorption, resonance Raman, and circular dichroism) and electrochemical methods. The results show that the thermal stability of the Au-cyt c superstructure biocomposite formed by the electrostatic and hydrophobic interactions among the associated proteins increases significantly. It is mainly caused by strong hydrophobicity of the associated cyt c in Au-cyt c superstructure at high temperature, which results from the compact secondary structure and the packing of hydrophobic side chains around the Trp 59 and heme. In addition, the formation of bis-His configuration of heme is facilitated by the tightly self-associated state of cyt c in the Au-cyt c superstructure. The electrostatic coupling of the opposite charges among shells of the adsorbed proteins due to the formation of the superstructure biocomposite can reduce repulsions among the same charges in protein. These factors are also important for enhancing the stability of the associated cyt c. Furthermore, the voltammetric behavior of Au-cyt c at DNA modified glassy carbon electrode has been investigated for extending the application of Au-cyt c.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 30 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 02 Sep 2014, 14:14:36 EST by System User on behalf of Learning and Research Services (UQ Library)