Biocompatibility and biodistribution of surface-enhanced raman scattering nanoprobes in zebrafish embryos: In vivo and multiplex imaging

Wang, Yuling, Seebald, Jamie L., Szeto, Daniel P. and Irudayaraj, Joseph (2010) Biocompatibility and biodistribution of surface-enhanced raman scattering nanoprobes in zebrafish embryos: In vivo and multiplex imaging. ACS Nano, 4 7: 4039-4053. doi:10.1021/nn100351h


Author Wang, Yuling
Seebald, Jamie L.
Szeto, Daniel P.
Irudayaraj, Joseph
Title Biocompatibility and biodistribution of surface-enhanced raman scattering nanoprobes in zebrafish embryos: In vivo and multiplex imaging
Journal name ACS Nano   Check publisher's open access policy
ISSN 1936-0851
1936-086X
Publication date 2010-07-27
Year available 2010
Sub-type Article (original research)
DOI 10.1021/nn100351h
Open Access Status
Volume 4
Issue 7
Start page 4039
End page 4053
Total pages 15
Place of publication Washington, DC United States
Publisher American Chemical Society
Collection year 2010
Language eng
Subject 2200 Engineering
2500 Materials Science
3100 Physics and Astronomy
Abstract Nanoparticles are increasingly being used to investigate biological processes in various animal models due to their versatile chemical, unique optical, and multifunctional properties. In this report we address the biocompatibility and biodistribution of nanoparticle sensors used for Raman chemical imaging in live zebrafish (Danio rerio) embryos. Surface-enhanced Raman scattering (SERS) nanoprobes (NPs) comprising gold nanoparticles (AuNPs) as enhancing substrate and nonfluorescent Raman labels were synthesized and microinjected into zebrafish embryos at the one-cell stage. Raman mapping was performed to assess their distribution in various cell-types and tissues of developing embryo at five different stages between 6 and 96 hpf (hours post-fertilization). Biocompatibility and toxicity studies indicate that the NPs are not toxic and the embryos were found to exhibit normal morphological and gene expression in addition to the proper form and function of major organs such as the heart and vasculature (of 7 day old NPs injected zebrafish embryos). A multiplex in vivo detection protocol was developed by SERS imaging to demonstrate that multiple labels can be detected by Raman mapping in undifferentiated cells as they develop into distinct cell- and tissue-types. The present work is the first to report on multiplex Raman imaging of zebrafish embryos with potential implications in tracking tissue development and biological processes at single molecule sensitivity using appropriate target molecules in vivo.
Keyword Biocompatibility
Biodistribution
Gene expression
Multiplex in vivo Raman mapping
Zebrafish embryos
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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