SERS driven cross-platform based multiplex pathogen detection

Ravindranath, Sandeep P., Wang, Yuling and Irudayaraj, Joseph (2011) SERS driven cross-platform based multiplex pathogen detection. Sensors and Actuators, B: Chemical, 152 2: 183-190. doi:10.1016/j.snb.2010.12.005

Author Ravindranath, Sandeep P.
Wang, Yuling
Irudayaraj, Joseph
Title SERS driven cross-platform based multiplex pathogen detection
Journal name Sensors and Actuators, B: Chemical   Check publisher's open access policy
ISSN 0925-4005
Publication date 2011-03-01
Year available 2011
Sub-type Article (original research)
DOI 10.1016/j.snb.2010.12.005
Open Access Status
Volume 152
Issue 2
Start page 183
End page 190
Total pages 8
Place of publication Lausanne, Switzerland
Publisher Elsevier S.A.
Collection year 2011
Language eng
Subject 3105 Instrumentation
2505 Materials Chemistry
2508 Surfaces, Coatings and Films
2506 Metals and Alloys
2504 Electronic, Optical and Magnetic Materials
3104 Condensed Matter Physics
2208 Electrical and Electronic Engineering
Abstract We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 102 and 103 CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples.
Keyword Aptamers
Cross platform detection
Multiplex detection
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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