Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang, Yuling, Salehi, Mohammad, Schutz, Max, Rudi, Katharina and Schlucker, Sebastian (2013) Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells. Analyst, 138 6: 1764-1771. doi:10.1039/c3an36610c

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Author Wang, Yuling
Salehi, Mohammad
Schutz, Max
Rudi, Katharina
Schlucker, Sebastian
Title Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells
Journal name Analyst   Check publisher's open access policy
ISSN 0003-2654
1364-5528
Publication date 2013-03-21
Year available 2013
Sub-type Article (original research)
DOI 10.1039/c3an36610c
Open Access Status File (Publisher version)
Volume 138
Issue 6
Start page 1764
End page 1771
Total pages 8
Place of publication Cambridge, United Kingdom
Publisher R S C Publications
Collection year 2013
Language eng
Subject 1602 Criminology
1607 Social Work
1603 Demography
1303 Specialist Studies in Education
2304 Environmental Chemistry
Abstract Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono- and tri-ethylene glycol (EG) spacers, respectively. The stability of the hydrophilically stabilized metal colloid was tested under different conditions. In contrast to metal colloids coated with a SAM without terminal EG spacers, the hydrophilically stabilized SERS particles do not aggregate under physiologically relevant conditions, i.e., buffer solutions with high ionic strength. Using these rationally designed SERS particles in conjunction with a microspectroscopic acquisition scheme, a sandwich immunoassay for the sensitive detection of interleukin-6 (IL-6) was developed. Several control experiments demonstrate the high specificity of the assay towards IL-6, with a lowest detectable concentration of ca. 1 pg mL -1. The signal strength of the Au/Ag-NS is at least one order of magnitude higher compared with hydrophilically stabilized, non-aggregated solid quasi-spherical Au-NPs of the same size. This journal is
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Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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