The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue

Matindoost, Leila, Jalali Sendi, Jalal, Soleimanjahi, Hoorieh, Etebari, Kayvan and Rahbarizadeh, Fateme (2008) The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue. In Vitro Cellular and Developmental Biology - Animal, 44 3-4: 121-127. doi:10.1007/s11626-008-9083-3


Author Matindoost, Leila
Jalali Sendi, Jalal
Soleimanjahi, Hoorieh
Etebari, Kayvan
Rahbarizadeh, Fateme
Title The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue
Formatted title
The effects of BmNPV on biochemical changes in primary cultures of Bombyx mori embryonic tissue 
Journal name In Vitro Cellular and Developmental Biology - Animal   Check publisher's open access policy
ISSN 1071-2690
1543-706X
Publication date 2008-02-21
Sub-type Article (original research)
DOI 10.1007/s11626-008-9083-3
Volume 44
Issue 3-4
Start page 121
End page 127
Total pages 7
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract The effect of Bombyx mori nuclear polyhedrosis virus (BmNPV) on biochemical changes of TC-100 medium containing 10% fetal bovine serum (FBS) in embryonic primary cultures of silkworm was investigated. The primary cultures that reached 60% confluence were infected by 0.5, 1, and 2-ml viral inoculums (diluted with TC-100 medium representing multiplicity of infection (MOI) of 0.25, 0.5, and 1). Glucose, uric acid, urea, total protein, cholesterol, and alkaline phosphatase were measured in the medium of BmNPV-infected primary cultures. All biochemical compounds showed significant changes. Glucose decreased considerably by about 55 mg/ml, while different concentrations of the virus inoculums did not demonstrate significant differences among them. Total protein had only increased in 2 ml concentration and there were no changes in other concentrations. Uric acid as a by-product accumulated dramatically in all concentrations, while the amount of urea reduced in all treatments and this reduction was more evident in lower concentrations. Cholesterol consumption was high in cultures postinfection, while alkaline phosphatase (ALP) activity decreased in infected cells.
Keyword Biochemical changes introduction
BmNPV
Bombyx mori
Insect cell
Primary culture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Mon, 01 Sep 2014, 19:46:48 EST by Kayvan Etebari Jirsaraei on behalf of School of Biological Sciences