Diazinon resistance in different selected strains of Chilo suppressalis (Lepidoptera: Crambidae) in northern Iran

Zibaee, Arash, Jalali Sendi, Jalal, Ghadamyari, Mohammad, Alinia, Faramarz and Etebari, Kayvan (2009) Diazinon resistance in different selected strains of Chilo suppressalis (Lepidoptera: Crambidae) in northern Iran. Journal of Economic Entomology, 102 3: 1189-1196. doi:10.1603/029.102.0343


Author Zibaee, Arash
Jalali Sendi, Jalal
Ghadamyari, Mohammad
Alinia, Faramarz
Etebari, Kayvan
Title Diazinon resistance in different selected strains of Chilo suppressalis (Lepidoptera: Crambidae) in northern Iran
Formatted title
Diazinon resistance in different selected strains of Chilo suppressalis (Lepidoptera: Crambidae) in northern Iran
Journal name Journal of Economic Entomology   Check publisher's open access policy
ISSN 0022-0493
1938-291X
Publication date 2009-06-01
Sub-type Article (original research)
DOI 10.1603/029.102.0343
Open Access Status
Volume 102
Issue 3
Start page 1189
End page 1196
Total pages 8
Place of publication Lanham, MD, United States
Publisher Entomological Society of America
Language eng
Abstract Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), is a cosmopolitan and destructive pest in rice fields of the world. This pest was reported in 1973 in Iran, and it has since spread widely in rice, Oryza sativa L., fields throughout the country. In this study, we tried to evaluate comparative toxicity of diazinon in five colonies of C. suppressalis, collected from Babol (Ba), Amol (Am) of Mazandaran Province and Rasht (Ra), Sheikhmahale (Sh), and Gourabzarmikh (Go) of Guilan Province, northern Iran. The LD 50 values were compared. We also evaluated the general esterases, alkaline phosphatase (ALP), glutathione transferase (GST), and acetylcholinesterase (AChE) activities from the five populations. The LD 50 values of Ra, Ba, Am, and Sh (12.64, 11.4, 7.17, and 3.71 μg/mg larva-1) were 13.67-, 12.33-, 7.75-, and 4.02-fold higher than Go population (0.924 μg/mg larva-1). Using α-naphthyl acetate as substrate, the general esterase activities in Ra, Ba, Am, and Sh colonies were, respectively, 1.81-, 1.68-, 1.75-, and 1.35-fold more than those in Go population. When β-naphthyl acetate was used as the substrate, activity ratio was measured 1.98-, 2.58-, 1.25-, and 1.24-fold compared with the Go population. Glutathione transferase activities in Ra, Ba, Am, and Sh populations were 1.27-, 1.68-, 0.98-, and 1.7-fold more than those in Go, when l-chloro-2,4-dinitrobenzene was used as the substrate. When 1,2-dichloro-4- nitro-benzene was used as the substrate, activity ratio was measured 1.14-, 1.42-, 0.56-, and 0.95-fold compared with Go population. The ALP activity demonstrated a significant difference among these populations and in Ra, Ba, Am, and Sh larvae were 3.54-, 4.62-, 3.84-, and 2.18-fold more than Go. The AChE inhibition or I50 value was 0.19, 0.22, 0.31, 0.19, and 0.26 mM in Ra, Ba, Am, Sh and Go populations, respectively. However, the results showed no significant differences in studied colonies. These biochemical characterizations of general esterases ALP, GST, and AChE were consistent with diazinon bioassay in the five populations. It is inferred from increased esterase, alkaline phosphatase and glutathione transferase, activities that might play an important role in the increasing resistance in C. suppressalis to diazinon among these five populations.
Keyword Chilo suppressalis
Alkaline phosphatase
Diazinon resistance genotypes
General esterases
Glutathione Transferases
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Mon, 01 Sep 2014, 09:37:57 EST by Kayvan Etebari Jirsaraei on behalf of School of Biological Sciences