Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging

Richards, Kay, Calamante, Fernando, Tournier, Jacques-Donald, Kurniawan, Nyoman D., Sadeghian, Farnoosh, Retchford, Alexander R., Jones, Gabriel Davis, Reid, Christopher A., Reutens, David C., Ordidge, Roger, Connelly, Alan and Petrou, Steven (2014) Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging. Neuroimage, 102 2: 381-392. doi:10.1016/j.neuroimage.2014.07.048

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Author Richards, Kay
Calamante, Fernando
Tournier, Jacques-Donald
Kurniawan, Nyoman D.
Sadeghian, Farnoosh
Retchford, Alexander R.
Jones, Gabriel Davis
Reid, Christopher A.
Reutens, David C.
Ordidge, Roger
Connelly, Alan
Petrou, Steven
Title Mapping somatosensory connectivity in adult mice using diffusion MRI tractography and super-resolution track density imaging
Journal name Neuroimage   Check publisher's open access policy
ISSN 1095-9572
1053-8119
Publication date 2014
Sub-type Article (original research)
DOI 10.1016/j.neuroimage.2014.07.048
Open Access Status File (Author Post-print)
Volume 102
Issue 2
Start page 381
End page 392
Total pages 12
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Abstract In this study we combined ultra-high field diffusion MRI fiber tracking and super-resolution track density imaging (TDI) to map the relay locations and connectivity of the somatosensory pathway in paraformaldehyde fixed, C57Bl/6J mouse brains. Super-resolution TDI was used to achieve 20. μm isotropic resolution to inform the 3D topography of the relay locations including thalamic barreloids and brainstem barrelettes, not described previously using MRI methodology. TDI-guided mapping results for thalamo-cortical connectivity were consistent with thalamo-cortical projections labeled using virus mediated fluorescent protein expression. Trigemino-thalamic TDI connectivity maps were concordant with results obtained using anterograde dye tracing from brainstem to thalamus. Importantly, TDI mapping overcame the constraint of tissue distortion observed in mechanically sectioned tissue, enabling 3D reconstruction and long-range connectivity data. In conclusion, our results showed that diffusion micro-imaging at ultra-high field MRI revealed the stereotypical pattern of somatosensory connectivity and is a valuable tool to complement histologic methods, achieving 3D spatial preservation of whole brain networks for characterization in mouse models of human disease.
Keyword Connectivity
Fiber tracking
Mouse brain
Somatosensory pathway
Track-density imaging
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Centre for Advanced Imaging Publications
 
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Created: Fri, 29 Aug 2014, 11:57:47 EST by Shona Osborne on behalf of Centre for Advanced Imaging