Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice

Aslam, Mohamad F., Frazer, David M., Faria, Nuno, Bruggraber, Sylvaine F. A., Wilkins, Sarah J., Mirciov, Cornel, Powell, Jonathan J., Anderson, Greg J. and Pereira, Dora I. A. (2014) Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice. FASEB Journal, 28 8: 3671-3678. doi:10.1096/fj.14-251520


Author Aslam, Mohamad F.
Frazer, David M.
Faria, Nuno
Bruggraber, Sylvaine F. A.
Wilkins, Sarah J.
Mirciov, Cornel
Powell, Jonathan J.
Anderson, Greg J.
Pereira, Dora I. A.
Title Ferroportin mediates the intestinal absorption of iron from a nanoparticulate ferritin core mimetic in mice
Journal name FASEB Journal   Check publisher's open access policy
ISSN 1530-6860
0892-6638
Publication date 2014-08
Year available 2014
Sub-type Article (original research)
DOI 10.1096/fj.14-251520
Open Access Status
Volume 28
Issue 8
Start page 3671
End page 3678
Total pages 8
Place of publication Bethesda, MD, United States
Publisher Federation of American Societies for Experimental Biology
Collection year 2015
Language eng
Formatted abstract
The ferritin core is composed of fine nanoparticulate Fe3+ oxohydroxide, and we have developed a synthetic mimetic, nanoparticulate Fe3+ polyoxohydroxide (nanoFe3+). The aim of this study was to determine how dietary iron derived in this fashion is absorbed in the duodenum. Following a 4 wk run-in on an Fe-deficient diet, mice with intestinal-specific disruption of the Fpn-1 gene (Fpn-KO), or littermate wild-type (WT) controls, were supplemented with Fe2+ sulfate (FeSO4), nanoFe3+, or no added Fe for a further 4 wk. A control group was Fe sufficient throughout. Direct intestinal absorption of nanoFe3+ was investigated using isolated duodenal loops. Our data show that FeSO4 and nanoFe3+ are equally bioavailable in WT mice, and at wk 8 the mean ± SEM hemoglobin increase was 18 ± 7 g/L in the FeSO4 group and 30 ± 5 g/L in the nanoFe3+ group. Oral iron failed to be utilized by Fpn-KO mice and was retained in enterocytes, irrespective of the iron source. In summary, although nanoFe3+ is taken up directly by the duodenum its homeostasis is under the normal regulatory control of dietary iron absorption, namely via ferroportin-dependent efflux from enterocytes, and thus offers potential as a novel oral iron supplement.
Keyword Nanoiron
Basolateral export
Iron homeostasis
Hepcidin
Knockout mice
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Chemistry and Molecular Biosciences
School of Medicine Publications
 
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