Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115)

Moffat, L., Rothwell, L., Garcia-Morales, C., Sauter, K. A., Kapetanovic, R., Gow, D. J. and Hume, D. A. (2014) Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115). Developmental and Comparative Immunology, 47 1: 123-128. doi:10.1016/j.dci.2014.07.001


Author Moffat, L.
Rothwell, L.
Garcia-Morales, C.
Sauter, K. A.
Kapetanovic, R.
Gow, D. J.
Hume, D. A.
Title Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115)
Journal name Developmental and Comparative Immunology   Check publisher's open access policy
ISSN 1879-0089
0145-305X
Publication date 2014-11
Year available 2014
Sub-type Letter to editor, brief commentary or brief communication
DOI 10.1016/j.dci.2014.07.001
Open Access Status
Volume 47
Issue 1
Start page 123
End page 128
Total pages 6
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Abstract Macrophage colony-stimulating factor (CSF1) controls the proliferation and differentiation of cells of the mononuclear phagocyte system. CSF1, alongside a second ligand, interleukin-34 (IL-34), acts by binding to a cell surface receptor (CSF1R). We previously cloned and expressed pig CSF1 and IL-34. Here we produced a pig CSF1R-Ig. +. pFUSE Fc fusion protein and used it as an immunogen to produce three monoclonal antibodies (ROS8G11, ROS3A5 and ROS3B10) targeted against porcine CSF1R. Specific binding of each monoclonal antibody was confirmed by ELISA, Western blot, flow cytometry and immunocytochemistry. The antibodies did not block CSF1 signalling. The surface expression of CSF1R in pig peripheral blood was restricted to CD14-positive monocytes and was also detected on lung macrophages. These antibodies provided an opportunity to investigate the increase of available CSF1R during pig BMDM differentiation. The new monoclonal antibodies provide useful reagents to support the study of monocyte and macrophage biology in the pig.
Keyword CSF1R
Porcine
Monoclonal antibodies
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collection: Institute for Molecular Bioscience - Publications
 
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