Role for lysosomal phospholipase A2 in iNKT cell-mediated CD1d recognition

Paduraru, Crina, Bezbradica, Jelena S., Kunte, Amit, Kelly, Robert, Shayman, James A., Veerapen, Natacha, Cox, Liam R., Besra, Gurdyal S. and Cresswell, Peter (2013) Role for lysosomal phospholipase A2 in iNKT cell-mediated CD1d recognition. Proceedings of the National Academy of Sciences of the United States of America, 110 13: 5097-5102. doi:10.1073/pnas.1302923110


Author Paduraru, Crina
Bezbradica, Jelena S.
Kunte, Amit
Kelly, Robert
Shayman, James A.
Veerapen, Natacha
Cox, Liam R.
Besra, Gurdyal S.
Cresswell, Peter
Title Role for lysosomal phospholipase A2 in iNKT cell-mediated CD1d recognition
Journal name Proceedings of the National Academy of Sciences of the United States of America   Check publisher's open access policy
ISSN 0027-8424
1091-6490
Publication date 2013-03-26
Sub-type Article (original research)
DOI 10.1073/pnas.1302923110
Open Access Status
Volume 110
Issue 13
Start page 5097
End page 5102
Total pages 6
Place of publication Washington, DC, United States
Publisher National Academy of Sciences
Language eng
Abstract Invariant natural killer T (iNKT) cells recognize self lipid antigens presented by CD1d molecules. The nature of the self-antigens involved in the development and maturation of iNKT cells is poorly defined. Lysophospholipids are self-antigens presented by CD1d that are generated through the action of phospholipases A1 and A2. Lysosomal phospholipase A2 (LPLA2, group XV phospholipase A2) resides in the endocytic system, the main site where CD1d antigen acquisition occurs, suggesting that it could be particularly important in CD1d function. We find that Lpla2-/- mice show a decrease in iNKT cell numbers that is neither the result of a general effect on the development of lymphocyte populations nor of effects on CD1d expression. However, endogenous lipid antigen presentation by CD1d is reduced in the absence of LPLA2. Our data suggest that LPLA2 plays a role in the generation of CD1d complexes with thymic lipids required for the normal selection and maturation of iNKT cells.
Keyword Antigen processing
Lipid storage disease
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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