A comparative study of the efficacy of intralesional verapamil versus normal saline injection in a novel peyronie disease animal model: assessment of immunohistopathological changes and erectile function outcome

Chung, Eric, Garcia, Francisco, De Young, Ling, Solomon, Matthew and Brock, Gerald B. (2013) A comparative study of the efficacy of intralesional verapamil versus normal saline injection in a novel peyronie disease animal model: assessment of immunohistopathological changes and erectile function outcome. Journal of Urology, 189 1: 380-384. doi:10.1016/j.juro.2012.08.191


Author Chung, Eric
Garcia, Francisco
De Young, Ling
Solomon, Matthew
Brock, Gerald B.
Title A comparative study of the efficacy of intralesional verapamil versus normal saline injection in a novel peyronie disease animal model: assessment of immunohistopathological changes and erectile function outcome
Journal name Journal of Urology   Check publisher's open access policy
ISSN 0022-5347
1527-3792
Publication date 2013
Sub-type Article (original research)
DOI 10.1016/j.juro.2012.08.191
Open Access Status
Volume 189
Issue 1
Start page 380
End page 384
Total pages 5
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
Purpose: While intralesional injections improve penile curvature and decrease plaque volume, the exact mechanism of action on Peyronie disease is unknown. We evaluated penile curvature, immunohistology and erectile function outcomes after intralesional injections of verapamil and normal saline in a previously described Peyronie disease animal model.

Materials and Methods: Peyronie plaque was induced in 12 adult male rats using an established Peyronie disease animal model. At 4 weeks the rats were divided into group 1 - 5 with 0.1 mg/0.1 ml intralesional verapamil injected every second day for 2 weeks, group 2 - 5 with 0.1 ml intralesional normal saline injection and group 3 - 2 that served as controls. At weeks 6 and 8 penile pressure was measured and serial immunohistochemical staining of penile tissue sections was done.

Results: Intralesional injection of verapamil and normal saline resulted in macroscopic and microscopic changes to penile curvature and Peyronie plaque size. Decreased collagen and elastin fibers were measured with a significant reduction in smooth muscle α-actin (p <0.05). Changes were greater in group 1 than group 2 (p <0.05). Intralesional verapamil injection was associated with greater recovery of electrostimulated penile pressure, a surrogate of erectile function, than in the saline and control groups.

Conclusions: To our knowledge this novel study offers for the first time histological evidence of cellular changes and improvement in penile pressure studies in rats with Peyronie plaque after intralesional verapamil injection therapy in a Peyronie disease animal model. 
Keyword Penile erection
Penile induration
Penis
Smooth muscle actin
Rat
Verapamil
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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