Genome-wide identification of miR-200 targets reveals a regulatory network controlling cell invasion

Bracken, Cameron P., Li, Xiaochun, Wright, Joesphine A., Lawrence, David, Pillman, Katherine A., Salmanidis, Markia, Anderson, Matthew A., Dredge, B. Kate, Gregory, Philip A., Tsykin, Anna, Neilsen, Corine, Thomson, Daniel W., Bert, Andrew G., Leerberg, Joanne M., Yap, Alpha S., Jensen, Kirk B., Khew-Goodall, Yeesim and Goodall, Gregory J. (2014) Genome-wide identification of miR-200 targets reveals a regulatory network controlling cell invasion. The EMBO Journal, 33 18: 2040-2056. doi:10.15252/embj.201488641

Author Bracken, Cameron P.
Li, Xiaochun
Wright, Joesphine A.
Lawrence, David
Pillman, Katherine A.
Salmanidis, Markia
Anderson, Matthew A.
Dredge, B. Kate
Gregory, Philip A.
Tsykin, Anna
Neilsen, Corine
Thomson, Daniel W.
Bert, Andrew G.
Leerberg, Joanne M.
Yap, Alpha S.
Jensen, Kirk B.
Khew-Goodall, Yeesim
Goodall, Gregory J.
Title Genome-wide identification of miR-200 targets reveals a regulatory network controlling cell invasion
Journal name The EMBO Journal   Check publisher's open access policy
ISSN 0261-4189
Publication date 2014-07-28
Year available 2014
Sub-type Article (original research)
DOI 10.15252/embj.201488641
Open Access Status
Volume 33
Issue 18
Start page 2040
End page 2056
Total pages 17
Place of publication Oxford, United Kingdom
Publisher Wiley-Blackwell Publishing Ltd.
Collection year 2015
Language eng
Formatted abstract
The microRNAs of the miR‐200 family maintain the central characteristics of epithelia and inhibit tumor cell motility and invasiveness. Using the Ago‐HITS‐CLIP technology for transcriptome‐wide identification of direct microRNA targets in living cells, along with extensive validation to verify the reliability of the approach, we have identified hundreds of miR‐200a and miR‐200b targets, providing insights into general features of miRNA target site selection. Gene ontology analysis revealed a predominant effect of miR‐200 targets in widespread coordinate control of actin cytoskeleton dynamics. Functional characterization of the miR‐200 targets indicates that they constitute subnetworks that underlie the ability of cancer cells to migrate and invade, including coordinate effects on Rho‐ROCK signaling, invadopodia formation, MMP activity, and focal adhesions. Thus, the miR‐200 family maintains the central characteristics of the epithelial phenotype by acting on numerous targets at multiple levels, encompassing both cytoskeletal effectors that control actin filament organization and dynamics, and upstream signals that locally regulate the cytoskeleton to maintain cell morphology and prevent cell migration.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 43 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 43 times in Scopus Article | Citations
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Created: Thu, 14 Aug 2014, 11:54:07 EST by Susan Allen on behalf of Institute for Molecular Bioscience