The novel OCT4 spliced variant OCT4B1 can generate three protein isoforms by alternative splicing into OCT4B

Gao, Yuan, Wang, Xia, Han, Jin, Xiao, Zhifeng, Chen, Bing, Su, Guannan and Dai, Jianwu (2010) The novel OCT4 spliced variant OCT4B1 can generate three protein isoforms by alternative splicing into OCT4B. Journal of Genetics and Genomics, 37 7: 461-465. doi:10.1016/S1673-8527(09)60065-5


Author Gao, Yuan
Wang, Xia
Han, Jin
Xiao, Zhifeng
Chen, Bing
Su, Guannan
Dai, Jianwu
Title The novel OCT4 spliced variant OCT4B1 can generate three protein isoforms by alternative splicing into OCT4B
Journal name Journal of Genetics and Genomics   Check publisher's open access policy
ISSN 1673-8527
1873-5533
Publication date 2010
Year available 2010
Sub-type Article (original research)
DOI 10.1016/S1673-8527(09)60065-5
Open Access Status
Volume 37
Issue 7
Start page 461
End page 465
Total pages 5
Place of publication Amsterdam, The Netherlands
Publisher Elsevier BV
Collection year 2010
Language eng
Subject 1311 Genetics
1312 Molecular Biology
Abstract OCT4 is one of the key transcription factors in maintaining the pluripotency and self-renewal of embryonic stem (ES) cells. Human OCT4 can generate two isoforms OCT4A and OCT4B by alternative splicing. OCT4B1 is a recently discovered novel OCT4 spliced variant, which has been considered as a putative marker of stemness. Compared with the OCT4B mRNA, OCT4B1 mRNA is generated by retaining intron 2 as a cryptic exon which contains a TGA stop codon in it. As a result, the protein product of OCT4B1 mRNA could be truncated. Interestingly, we present here that OCT4B1 can indirectly produce the same protein products as OCT4B. We have demonstrated that OCT4B1 mRNA can be spliced into OCT4B mRNA, and encode three protein isoforms. The splicing of OCT4B1 mRNA into OCT4B mRNA can be remarkably inhibited by the mutation of the classical splicing site. Our result suggests that OCT4B mRNA may originate from OCT4B1 mRNA by alternative splicing.
Keyword Alternative splicing
Alternative translation initiation
Stress
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 14 Aug 2014, 10:26:53 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology