Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism

Khalil, Zeinab G., Kalansuriya, Pabasara and Capon, Robert J. (2014) Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism. Mycology, 5 3: 168-178. doi:10.1080/21501203.2014.930530

Author Khalil, Zeinab G.
Kalansuriya, Pabasara
Capon, Robert J.
Title Lipopolysaccharide (LPS) stimulation of fungal secondary metabolism
Journal name Mycology   Check publisher's open access policy
ISSN 2150-1211
Publication date 2014
Sub-type Article (original research)
DOI 10.1080/21501203.2014.930530
Open Access Status DOI
Volume 5
Issue 3
Start page 168
End page 178
Total pages 11
Place of publication Abingdon, Oxfordshire, United Kingdom
Publisher Taylor and Francis
Collection year 2015
Abstract We report on a preliminary investigation of the use the Gram-negative bacterial cell wall constituent lipopolysaccharide (LPS) as a natural chemical cue to stimulate and alter the expression of fungal secondary metabolism. Integrated high-throughput micro-cultivation and micro-analysis methods determined that 6 of 40 (15%) of fungi tested responded to an optimal exposure to LPS (0.6 ng/mL) by activating, enhancing or accelerating secondary metabolite production. To explore the possible mechanisms behind this effect, we employed light and fluorescent microscopy in conjunction with a nitric oxide (NO)-sensitive fluorescent dye and an NO scavenger to provide evidence that LPS stimulation of fungal secondary metabolism coincided with LPS activation of NO. Several case studies demonstrated that LPS stimulation can be scaled from single microplate well (1.5 mL) to preparative (>400 mL) scale cultures. For example, LPS treatment of Penicillium sp. (ACM-4616) enhanced pseurotin A and activated pseurotin A1 and pseurotin A2 biosynthesis, whereas LPS treatment of Aspergillus sp. (CMB-M81F) substantially accelerated and enhanced the biosynthesis of shornephine A and a series of biosynthetically related ardeemins and activated production of neoasterriquinone. As an indication of broader potential, we provide evidence that cultures of Penicillium sp. (CMB-TF0411), Aspergillus niger (ACM-4993F), Rhizopus oryzae (ACM-165F) and Thanatephorus cucumeris (ACM-194F) were responsive to LPS stimulation, the latter two examples being particular noteworthy as neither are known to produce secondary metabolites. Our results encourage the view that LPS stimulation can be used as a valuable tool to expand the molecular discovery potential of fungal strains that either have been exhaustively studied by or are unresponsive to traditional culture methodology. © 2014
Keyword Acceleration
Silent secondary metabolism
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
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