A comprehensive study of ovine haemostasis to assess suitability to model human coagulation

Foley, Samuel R., Solano, Connie, Simonova, Gabriela, Spanevello, Michelle M., Bird, Robert J., Semple, John W., Jackson, Denise E., Schibler, Andreas, Fraser, John F. and Fung, Yoke Lin (2014) A comprehensive study of ovine haemostasis to assess suitability to model human coagulation. Thrombosis Research, 134 2: 468-473. doi:10.1016/j.thromres.2014.05.026


Author Foley, Samuel R.
Solano, Connie
Simonova, Gabriela
Spanevello, Michelle M.
Bird, Robert J.
Semple, John W.
Jackson, Denise E.
Schibler, Andreas
Fraser, John F.
Fung, Yoke Lin
Title A comprehensive study of ovine haemostasis to assess suitability to model human coagulation
Journal name Thrombosis Research   Check publisher's open access policy
ISSN 1879-2472
0049-3848
Publication date 2014-08
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.thromres.2014.05.026
Open Access Status
Volume 134
Issue 2
Start page 468
End page 473
Total pages 6
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Collection year 2015
Language eng
Formatted abstract
Introduction

Similarities in size, anatomy and physiology have supported the use of sheep to model a wide range of human diseases, including coagulopathy. However, coagulation studies involving sheep are limited by the absence of high quality data defining normal ovine coagulation and fibrinolysis.

Materials and Methods

Full blood examination, routine and specialised coagulation tests, rotational thromboelastometry and whole blood platelet aggregometry was performed on 50 healthy Samm & Border Leicester Cross ewes and compared to corresponding human ranges. Intraspecies breed and gender variability was investigated by comparison to a smaller population of 13 healthy Merino wethers.

Results

Ovine coagulation was similar to human according to routine coagulation methods (PT, aPTT, TCT, Fib(C)) and some specialised coagulation tests (vWF, AT, Plasmin Inh). Despite these similarities, ovine secondary haemostasis demonstrated substantial differences to that of human. Rapid initiation of the contact activation pathway, high levels of FVIII, low Protein C, greater overall clot firmness and a reduced capacity for clot lysis was documented in sheep. In addition, ADP and collagen agonists precipitated a reduced primary haemostatic response in sheep relative to human. Intraspecies differences in whole blood platelet aggregometry between the cohorts of sheep indicate the need for breed-specific normal ranges.

Conclusions

The application of a board spectrum of coagulation assays has enabled elucidation of the similarities as well as differences between ovine and human coagulation. The new knowledge generated from this study will guide the design of future translational coagulation studies in ovine models.
Keyword Animal
Coagulation parameters
Normal values
Platelets
Sheep
Thromboelastometry
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Health Services Publications
Official 2015 Collection
School of Medicine Publications
 
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