Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle

Canovas, Angela, Reverter, Antonio, DeAtley, Kasey L., Ashley, Ryan L., Colgrave, Michelle L., Fortes, Marina R. S., Islas-Trejo, Alma, Lehnert, Sigrid, Porto-Neto, Laercio, Rincon, Gonzalo, Silver, Gail A., Snelling, Warren M., Medrano, Juan F. and Thomas, Milton G. (2014) Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. PLoS ONE, 9 7: . doi:10.1371/journal.pone.0102551


Author Canovas, Angela
Reverter, Antonio
DeAtley, Kasey L.
Ashley, Ryan L.
Colgrave, Michelle L.
Fortes, Marina R. S.
Islas-Trejo, Alma
Lehnert, Sigrid
Porto-Neto, Laercio
Rincon, Gonzalo
Silver, Gail A.
Snelling, Warren M.
Medrano, Juan F.
Thomas, Milton G.
Title Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-07
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0102551
Open Access Status DOI
Volume 9
Issue 7
Total pages 17
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2015
Language eng
Formatted abstract
Puberty is a complex physiological event by which animals mature into an adult capable of sexual reproduction. In order to enhance our understanding of the genes and regulatory pathways and networks involved in puberty, we characterized the transcriptome of five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, and endometrium) as well as tissues known to be relevant to growth and metabolism needed to achieve puberty (i.e., longissimus dorsi muscle, adipose, and liver). These tissues were collected from pre- and post-pubertal Brangus heifers (3/8 Brahman; Bos indicus x 5/8 Angus; Bos taurus) derived from a population of cattle used to identify quantitative trait loci associated with fertility traits (i.e., age of first observed corpus luteum (ACL), first service conception (FSC), and heifer pregnancy (HPG)). In order to exploit the power of complementary omics analyses, pre- and post-puberty co-expression gene networks were constructed by combining the results from genome-wide association studies (GWAS), RNA-Seq, and bovine transcription factors. Eight tissues among pre-pubertal and post-pubertal Brangus heifers revealed 1,515 differentially expressed and 943 tissue-specific genes within the 17,832 genes confirmed by RNA-Seq analysis. The hypothalamus experienced the most notable up-regulation of genes via puberty (i.e., 204 out of 275 genes). Combining the results of GWAS and RNA-Seq, we identified 25 loci containing a single nucleotide polymorphism (SNP) associated with ACL, FSC, and (or) HPG. Seventeen of these SNP were within a gene and 13 of the genes were expressed in uterus or endometrium. Multi-tissue omics analyses revealed 2,450 co-expressed genes relative to puberty. The pre-pubertal network had 372,861 connections whereas the post-pubertal network had 328,357 connections. A sub-network from this process revealed key transcriptional regulators (i.e., PITX2, FOXA1, DACH2, PROP1, SIX6, etc.). Results from these multi-tissue omics analyses improve understanding of the number of genes and their complex interactions for puberty in cattle.
Keyword Puberty
Composite beef cattle
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article ID: e102551

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 10 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 12 Aug 2014, 00:25:35 EST by System User on behalf of Qld Alliance for Agriculture and Food Innovation