Root defense analysis against fusarium oxysporum reveals new regulators to confer resistance

Chen, Yi Chung, Wong, Chin Lin, Muzzi, Frederico, Vlaardingerbroek, Ido, Kidd, Brendan N. and Schenk, Peter M. (2014) Root defense analysis against fusarium oxysporum reveals new regulators to confer resistance. Scientific Reports, 4 . doi:10.1038/srep05584

Author Chen, Yi Chung
Wong, Chin Lin
Muzzi, Frederico
Vlaardingerbroek, Ido
Kidd, Brendan N.
Schenk, Peter M.
Title Root defense analysis against fusarium oxysporum reveals new regulators to confer resistance
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2014-07-07
Year available 2014
Sub-type Article (original research)
DOI 10.1038/srep05584
Open Access Status DOI
Volume 4
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2015
Language eng
Formatted abstract
Fusarium oxysporum is a root-infecting fungal pathogen that causes wilt disease on a broad range of plant species, including Arabidopsis thaliana. Investigation of the defense response against this pathogen had primarily been conducted using leaf tissue and little was known about the root defense response. In this study, we profiled the expression of root genes after infection with F. oxysporum by microarray analysis. In contrast to the leaf response, root tissue did not show a strong induction of defense-associated gene expression and instead showed a greater proportion of repressed genes. Screening insertion mutants from differentially expressed genes in the microarray uncovered a role for the transcription factor ETHYLENE RESPONSE FACTOR72 (ERF72) in susceptibility to F. oxysporum. Due to the role of ERF72 in suppressing programmed cell death and detoxifying reactive oxygen species (ROS), we examined the pub22/pub23/pub24 U-box type E3 ubiquitin ligase triple mutant which is known to possess enhanced ROS production in response to pathogen challenge. We found that the pub22/23/24 mutant is more resistant to F. oxysporum infection, suggesting that a heightened innate immune response provides protection against F. oxysporum. We conclude that root-mediated defenses against soil-borne pathogens can be provided at multiple levels.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
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Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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