Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity

Gosalvez, Jaime, Johnston, Stephen, Lopez-Fernandez, Carmen, Gosalbez, Altea, Arroyo, Francisca, Fernandez, Jose Luis and Alvarez, Juan G. (2014) Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity. Asian Pacific Journal of Reproduction, 3 2: 116-120. doi:10.1016/S2305-0500(14)60012-2

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Author Gosalvez, Jaime
Johnston, Stephen
Lopez-Fernandez, Carmen
Gosalbez, Altea
Arroyo, Francisca
Fernandez, Jose Luis
Alvarez, Juan G.
Title Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity
Journal name Asian Pacific Journal of Reproduction   Check publisher's open access policy
ISSN 2305-0500
2305-0519
Publication date 2014-06-20
Sub-type Article (original research)
DOI 10.1016/S2305-0500(14)60012-2
Open Access Status DOI
Volume 3
Issue 2
Start page 116
End page 120
Total pages 5
Place of publication WanChai, Hong Kong
Publisher Hongkong Gangmao Group
Collection year 2015
Language eng
Formatted abstract
Objective: To investigate the DNA longevity characteristics associated with each resultant fraction following density gradient centrifugation (DGC) in comparison to that of the original neat ejaculated sample.

Methods: An aliquot of neat semen (NSS) collected from 7 patients was processed using DGC resulting in 3 fractions; Fraction 1: seminal plasma/40% gradient  interface (GI); Fraction 2: 40%GI/80%GI; Fraction 3: 80%GI/pellet. An aliquot of each fraction
and NSS was cryopreserved, thawed and incubated at 37 C for 24h; the increase of sperm  DNA fragmentation was assessed using the Dyn-Halosperm assay following 0, 3, 6 and 24h of incubation.

Results: While there was a significant reduction in the incidence of baseline  sperm DNA fragmentation following DGC in Fraction 3, sperm DNA longevity was shown to be  higher in the NSS than in any other sub-population following incubation. The highest levels of  baseline DNA damage were found in Fractions 1 and 2; these fractions also showed the highest  rate DNA fragmentation following incubation, subsequently exhibiting the lowest DNA longevity.

Conclusion: 1) Unnecessary incubation of spermatozoa prior to artificial insemination or in vitro fertilization, should be avoided, since sperm DNA longevity is significantly reduced after ex vivo sperm handling and 2) Although sperm selection by DCG significantly reduces the baseline levels of SDF of sperm in Fraction 3, sperm DNA longevity in this fraction was ultimately lower following 24 h incubation when compared to sperm recovered from non-centrifuged NSS.
Keyword Sperm
Ejaculates
DNA longevity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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Created: Fri, 08 Aug 2014, 17:46:58 EST by Associate Professor Stephen Johnston on behalf of School of Agriculture and Food Sciences