Sperm DNA Fragmentation and its role in wildlife conservation

Gosalvez, Jaime, Holt, William V. and Johnston, Stephen D. (2014). Sperm DNA Fragmentation and its role in wildlife conservation. In William V. Holt, Janine L. Brown and Pierre Comizzoli (Ed.), Reproductive sciences in animal conservation: Progress and prospects (pp. 357-384) New York, NY United States: Springer. doi:10.1007/978-1-4939-0820-2-15

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Author Gosalvez, Jaime
Holt, William V.
Johnston, Stephen D.
Title of chapter Sperm DNA Fragmentation and its role in wildlife conservation
Title of book Reproductive sciences in animal conservation: Progress and prospects
Place of Publication New York, NY United States
Publisher Springer
Publication Year 2014
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-0820-2-15
Open Access Status
Year available 2014
Series Advances in Experimental Medicine and Biology
ISBN 9781493908196
9781493908202
ISSN 0065-2598
Editor William V. Holt
Janine L. Brown
Pierre Comizzoli
Volume number 753
Chapter number 15
Start page 357
End page 384
Total pages 28
Total chapters 20
Collection year 2015
Language eng
Formatted Abstract/Summary
Until about 20 years ago, sperm assessment in the laboratory was focused on motility, morphology and acrosomal integrity. Then came the gradual realisation that, because the main objective of a spermatozoon is to deliver an intact genetic payload of DNA to the egg, being able to check DNA quality of spermatozoa would be equally important, if not more so. Research over the last two decades has therefore led to the development of several techniques for reliably detecting DNA strand breaks, and the more recent focus has been directed towards understanding the fertility implications of DNA damage. It is now clear that evolutionary history has played an important role in determining the stability of sperm DNA under stressful conditions, and that the nature of the DNA-protein interactions also influence the extent to which fertility is affected by both technical procedures involved in sperm preservation and the basic biology of the species concerned. Here we present an overview of the principles involved in DNA assessment and also provide some cases studies that illustrate the influences of species diversity.
Keyword Cryopreservation
Spermatozoa
Rhinoceros
Elephant
Koala
Echidna
Donkey
Planigale
Q-Index Code B1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Book Chapter
Collections: School of Agriculture and Food Sciences
Official 2015 Collection
 
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Created: Fri, 08 Aug 2014, 17:29:35 EST by Associate Professor Stephen Johnston on behalf of School of Agriculture and Food Sciences