FimR and FimS: Biofilm formation and gene expression in Porphyromonas gingivalis

Lo, Alvin, Seers, Christine, Dashper, Stuart, Butler, Catherine, Walker, Glenn, Walsh, Katrina, Catmull, Deanne, Hoffmann, Brigitte, Cleal, Steven, Lissel, Patricia, Boyce, John and Reynolds, Eric (2010) FimR and FimS: Biofilm formation and gene expression in Porphyromonas gingivalis. Journal of Bacteriology, 192 5: 1332-1343. doi:10.1128/JB.01211-09

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Author Lo, Alvin
Seers, Christine
Dashper, Stuart
Butler, Catherine
Walker, Glenn
Walsh, Katrina
Catmull, Deanne
Hoffmann, Brigitte
Cleal, Steven
Lissel, Patricia
Boyce, John
Reynolds, Eric
Title FimR and FimS: Biofilm formation and gene expression in Porphyromonas gingivalis
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
Publication date 2010
Year available 2010
Sub-type Article (original research)
DOI 10.1128/JB.01211-09
Open Access Status File (Publisher version)
Volume 192
Issue 5
Start page 1332
End page 1343
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2010
Language eng
Abstract Porphyromonas gingivalis is a late-colonizing bacterium of the subgingival dental plaque biofilm associated with periodontitis. Two P. gingivalis genes, fimR and fimS, are predicted to encode a two-component signal transduction system comprising a response regulator (FimR) and a sensor histidine kinase (FimS). In this study, we show that fimS and fimR, although contiguous on the genome, are not part of an operon. We inactivated fimR and fimS in both the afimbriated strain W50 and the fimbriated strain ATCC 33277 and demonstrated that both mutants formed significantly less biofilm than their respective wild-type strains. Quantitative reverse transcription-real-time PCR showed that expression of fimbriation genes was reduced in both the fimS and fimR mutants of strain ATCC 33277. The mutations had no effect, in either strain, on the P. gingivalis growth rate or on the response to hydrogen peroxide or growth at pH 9, at 41° C, or at low hemin availability. Transcriptome analysis using DNA microarrays revealed that inactivation of fimS resulted in the differential expression of 10% of the P. gingivalis genome (>1.5-fold; P < 0.05). Notably genes encoding seven different transcriptional regulators, including the fimR gene and three extracytoplasmic sigma factor genes, were differentially expressed in the fimS mutant. Copyright
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Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Created: Thu, 07 Aug 2014, 12:53:48 EST by Ms Kate Rowe on behalf of School of Chemistry & Molecular Biosciences