A novel integrative conjugative element mediates genetic transfer from group g streptococcus to other ß-hemolytic streptococci

Davies, Mark R., Shera, Josephine, Van Domselaar, Gary H., Sriprakash, Kadaba S. and McMillan, David J. (2009) A novel integrative conjugative element mediates genetic transfer from group g streptococcus to other ß-hemolytic streptococci. Journal of Bacteriology, 191 7: 2257-2265. doi:10.1128/JB.01624-08

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Author Davies, Mark R.
Shera, Josephine
Van Domselaar, Gary H.
Sriprakash, Kadaba S.
McMillan, David J.
Title A novel integrative conjugative element mediates genetic transfer from group g streptococcus to other ß-hemolytic streptococci
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
Publication date 2009
Sub-type Article (original research)
DOI 10.1128/JB.01624-08
Open Access Status File (Publisher version)
Volume 191
Issue 7
Start page 2257
End page 2265
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Lateral gene transfer is a significant contributor to the ongoing evolution of many bacterial pathogens, including ß-hemolytic streptococci. Here we provide the first characterization of a novel integrative conjugative element (ICE), ICESde3396, from Streptococcus dysgalactiae subsp. equisimilis (group G streptococcus [GGS]), a bacterium commonly found in the throat and skin of humans. ICESde3396 is 64 kb in size and encodes 66 putative open reading frames. ICESde3396 shares 38 open reading frames with a putative ICE from Strepto- coccus agalactiae (group B streptococcus [GBS]), ICESa2603. In addition to genes involves in conjugal pro- cesses, ICESde3396 also carries genes predicted to be involved in virulence and resistance to various metals. A major feature of ICESde3396 differentiating it from ICESa2603 is the presence of an 18-kb internal recombinogenic region containing four unique gene clusters, which appear to have been acquired from streptococcal and nonstreptococcal bacterial species. The four clusters include two cadmium resistance oper- ons, an arsenic resistance operon, and genes with orthologues in a group A streptococcus (GAS) prophage. Streptococci that naturally harbor ICESde3396 have increased resistance to cadmium and arsenate, indicating the functionality of genes present in the 18-kb recombinogenic region. By marking ICESde3396 with a kanamycin resistance gene, we demonstrate that the ICE is transferable to other GGS isolates as well as GBS and GAS. To investigate the presence of the ICE in clinical streptococcal isolates, we screened 69 isolates (30 GGS, 19 GBS, and 20 GAS isolates) for the presence of three separate regions of ICESde3396. Eleven isolates possessed all three regions, suggesting they harbored ICESde3396-like elements. Another four isolates pos- sessed ICESa2603-like elements. We propose that ICESde3396 is a mobile genetic element that is capable of acquiring DNA from multiple bacterial sources and is a vehicle for dissemination of this DNA through the wider ß-hemolytic streptococcal population.
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Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 36 times in Thomson Reuters Web of Science Article | Citations
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