In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy

Ye, Shuji, Nguyen, Khoi Tan, Le Clair, Stéphanie V. and Chen, Zhan (2009) In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy. Journal of Structural Biology, 168 1: 61-77. doi:10.1016/j.jsb.2009.03.006


Author Ye, Shuji
Nguyen, Khoi Tan
Le Clair, Stéphanie V.
Chen, Zhan
Title In situ molecular level studies on membrane related peptides and proteins in real time using sum frequency generation vibrational spectroscopy
Journal name Journal of Structural Biology   Check publisher's open access policy
ISSN 1047-8477
1095-8657
Publication date 2009
Sub-type Article (original research)
DOI 10.1016/j.jsb.2009.03.006
Open Access Status
Volume 168
Issue 1
Start page 61
End page 77
Total pages 17
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Language eng
Abstract Sum frequency generation (SFG) vibrational spectroscopy has been demonstrated to be a powerful technique to study the molecular structures of surfaces and interfaces in different chemical environments. This review summarizes recent SFG studies on hybrid bilayer membranes and substrate-supported lipid monolayers and bilayers, the interaction between peptides/proteins and lipid monolayers/bilayers, and bilayer perturbation induced by peptides/proteins. To demonstrate the ability of SFG to determine the orientations of various secondary structures, studies on the interactions between different peptides/proteins (melittin, G proteins, alamethicin, and tachyplesin I) and lipid bilayers are discussed. Molecular level details revealed by SFG in these studies show that SFG can provide a unique understanding on the interactions between a lipid monolayer/bilayer and peptides/proteins in real time, in situ and without any exogenous labeling.
Keyword Alamethicin
Antimicrobial peptide
Cell membrane
G proteins
Lipid bilayer
Melittin
Membrane protein
SFG
Sum frequency generation
Tachyplesin I
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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