Comparative biomarker expression and RNA integrity in biospecimens derived from radical retropubic and robot-assisted laparoscopic prostatectomies

Ricciardelli, Carmela, Bianco-Miotto, Tina, Jindal, Shalini, Dodd, Thomas J., Cohen, Penelope A., Marshall, Villis R., Sutherland, Peter D., Samaratunga, Hemamali, Kench, James G., Dong, Ying, Wang, Hong, Clements, Judith A., Risbridger, Gail P., Sutherland, Robert L., Tilley, Wayne D. and Horsfall, David J. (2010) Comparative biomarker expression and RNA integrity in biospecimens derived from radical retropubic and robot-assisted laparoscopic prostatectomies. Cancer Epidemiology Biomarkers and Prevention, 19 7: 1755-1765. doi:10.1158/1055-9965.EPI-10-0059


Author Ricciardelli, Carmela
Bianco-Miotto, Tina
Jindal, Shalini
Dodd, Thomas J.
Cohen, Penelope A.
Marshall, Villis R.
Sutherland, Peter D.
Samaratunga, Hemamali
Kench, James G.
Dong, Ying
Wang, Hong
Clements, Judith A.
Risbridger, Gail P.
Sutherland, Robert L.
Tilley, Wayne D.
Horsfall, David J.
Title Comparative biomarker expression and RNA integrity in biospecimens derived from radical retropubic and robot-assisted laparoscopic prostatectomies
Journal name Cancer Epidemiology Biomarkers and Prevention   Check publisher's open access policy
ISSN 1055-9965
1538-7755
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1158/1055-9965.EPI-10-0059
Volume 19
Issue 7
Start page 1755
End page 1765
Total pages 11
Place of publication Philadelphia, PA United States
Publisher American Association for Cancer Research
Collection year 2010
Language eng
Subject 2713 Epidemiology
2730 Oncology
Abstract Background: Knowledge of preanalytic conditions that biospecimens are subjected to is critically important because novel surgical procedures, tissue sampling, handling, and storage might affect biomarker expression or invalidate tissue samples as analytes for some technologies. Methods: We investigated differences in RNA quality, gene expression by quantitative real-time PCR, and immunoreactive protein expression of selected prostate cancer biomarkers between tissues from retropubic radical prostatectomy(RRP) and robot-assisted laparoscopic prostatectomy (RALP). Sections of tissue microarray of 23 RALP and 22 RRP samples were stained with antibodies to androgen receptor (AR) and prostate-specific antigen (PSA) as intersite controls, and 14 other candidate biomarkers of research interest to three laboratories within the Australian Prostate Cancer BioResource tissue banking network. Quantitative real-time PCRwas done for AR, PSA (KLK3), KLK2, KLK4, and HIF1A on RNAextracted from five RALP and five RRP frozen tissue cores. Results: No histologic differences were observed between RALP and RRP tissue. Biomarker staining grouped these samples into those with increased (PSA, CK8/18, CKHMW, KLK4), decreased (KLK2, KLK14), or no change in expression (AR, ghrelin, Ki67, PCNA, VEGF-C, PAR2, YB1, p63, versican, and chondroitin 0-sulfate) in RALP compared with RRP tissue. No difference in RNA quality or gene expression was detected between RALP and RRP tissue. Conclusions: Changes in biomarker expression between RALP and RRP tissue exist at the immunoreactive protein level, but the etiology is unclear. Impact: Future studies should account for changes in biomarker expression when using RALP tissues, and mixed cohorts of RALP and RRP tissue should be avoided.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Sustainable Minerals Institute Publications
 
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