A comparison of convergently evolved insect silks that share β-sheet molecular structure

Church, Jeffrey S., Woodhead, Andrea L., Walker, Andrew A. and Sutherland, Tara D. (2014) A comparison of convergently evolved insect silks that share β-sheet molecular structure. Biopolymers, 101 6: 630-639. doi:10.1002/bip.22431


Author Church, Jeffrey S.
Woodhead, Andrea L.
Walker, Andrew A.
Sutherland, Tara D.
Title A comparison of convergently evolved insect silks that share β-sheet molecular structure
Journal name Biopolymers   Check publisher's open access policy
ISSN 1097-0282
0006-3525
Publication date 2014-06
Sub-type Article (original research)
DOI 10.1002/bip.22431
Open Access Status
Volume 101
Issue 6
Start page 630
End page 639
Total pages 10
Place of publication Hoboken United States
Publisher John Wiley & Sons
Collection year 2015
Language eng
Abstract Raspy crickets produce silk webs that are used to build shelters. These webs have been found to consist of both fiber and film components. Raman spectra obtained from both components were found to be very similar for a given species. The protein structure of the fibers and films produced by both species was predominately β-sheet with lesser amounts of β-turns, unordered and α-helical protein also detected. The orientation of the β-sheet backbone in the fiber was determined to be parallel to the fiber axis. Compared to cocoon and dragline silk the orientation distribution exhibits a significant randomly orientated protein component. Amino acid analysis confirmed the presence of glycine, serine, and alanine in both species, which are known to form antiparallel β-sheet structures. Both species, although at significantly different concentrations, where found to contain proline. This amino acid is uncommon in insect silks, and likely involved in increasing fiber elasticity.
Keyword Amino acid composition
Protein conformation
Protein orientation
Raman spectroscopy
Silk
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Institute for Molecular Bioscience - Publications
 
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