Cowpea mosaic virus-based expression of antigens and antibodies in plants

Sainsbury, Frank, Liu, Li and Lomonossoff, George P. (2009). Cowpea mosaic virus-based expression of antigens and antibodies in plants. In Recombinant pharmaceutical proteins from plants (pp. 25-39) Totowa, NJ, United States: Humana Press. doi:10.1007/978-1-59745-407-0_2


Author Sainsbury, Frank
Liu, Li
Lomonossoff, George P.
Title of chapter Cowpea mosaic virus-based expression of antigens and antibodies in plants
Title of book Recombinant pharmaceutical proteins from plants
Place of Publication Totowa, NJ, United States
Publisher Humana Press
Publication Year 2009
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-59745-407-0_2
Open Access Status
Series Methods in Molecular Biology
ISBN 9781588299789
9781597454070
ISSN 1064-3745
Volume number 483
Chapter number 2
Start page 25
End page 39
Total pages 15
Language eng
Abstract/Summary This chapter describes the use of Cowpea mosaic virus-based vectors for the production of foreign proteins such as antigens and antibodies in plants. The systems include vectors based on both full-length and deleted versions of RNA-2. In both cases, the modified RNA-2 is replicated by coinoculation with RNA-1. The constructs based on full-length RNA-2 retain the ability to spread systemically throughout an inoculated plant and the infection can be passaged. The vector based on a deleted version of RNA-2 can stably incorporate larger inserts but lacks the ability to move systemically. However, it has the added advantage of biocontainment. In both cases, vector constructs modified to contain a foreign gene of interest can be delivered by agroinfiltration to obtain transient expression of the foreign protein. If required, the same constructs can also be used for stable nuclear transformation. Both types of vector have proved effective for the production in plants of a diverse range of proteins including antigens and antibodies.
Keyword Agroinfiltration
CPMV
Deleted viral vector
Full-length viral vector
Suppressor of silencing
Virus passaging
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Tue, 05 Aug 2014, 15:34:20 EST by Cathy Fouhy on behalf of Aust Institute for Bioengineering & Nanotechnology