Rabbit liver growth hormone receptor and serum binding protein: purification, characterization, and sequence

Spencer, Steven A., Hammonds, R. Glenn, Henzel, William J., Rodriguez, Henry, Waters, Michael J. and Wood, William I. (1988) Rabbit liver growth hormone receptor and serum binding protein: purification, characterization, and sequence. Journal of Biological Chemistry, 263 16: 7862-7867.

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Author Spencer, Steven A.
Hammonds, R. Glenn
Henzel, William J.
Rodriguez, Henry
Waters, Michael J.
Wood, William I.
Title Rabbit liver growth hormone receptor and serum binding protein: purification, characterization, and sequence
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 1988-06-05
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 263
Issue 16
Start page 7862
End page 7867
Total pages 6
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Formatted abstract
A putative growth hormone receptor from detergent-solubilized rabbit liver membranes and the growth hormone binding protein from rabbit serum have been purified 59,000- and 400,000-fold, respectively, primarily by affinity chromatography. Both purified proteins exhibit high affinity binding for human growth hormone; Kα = 9-30 x 109 M-1 for the liver receptor and Kα = 6 x 109 M-1 for the binding protein. The apparent molecular weight of the liver receptor is 130,000 by reduced sodium dodecyl sulfate gel electrophoresis, while that of the binding protein is 51,000. Both contain N-linked carbohydrate. The amino-terminal sequences of the liver growth hormone receptor and the serum binding protein were found to be the same, indicating that the binding protein corresponds to the extracellular domain of the liver receptor. Ubiquitin was found covalently linked to the liver receptor but not to the serum binding protein. The amino acid sequences of several peptides from the liver receptor were also determined after tryptic and V8 protease digestion.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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