Expression vectors encoding human growth hormone (hGH) controlled by human muscle-specific promoters: prospects for regulated production of hGH delivered by myoblast transfer or intravenous injection

Dahler, Alison, Wade, Robert P., Muscat, George E. O. and Waters, Michael J. (1994) Expression vectors encoding human growth hormone (hGH) controlled by human muscle-specific promoters: prospects for regulated production of hGH delivered by myoblast transfer or intravenous injection. Gene, 145 2: 305-310. doi:10.1016/0378-1119(94)90025-6


Author Dahler, Alison
Wade, Robert P.
Muscat, George E. O.
Waters, Michael J.
Title Expression vectors encoding human growth hormone (hGH) controlled by human muscle-specific promoters: prospects for regulated production of hGH delivered by myoblast transfer or intravenous injection
Journal name Gene   Check publisher's open access policy
ISSN 0378-1119
1879-0038
Publication date 1994-08-05
Sub-type Article (original research)
DOI 10.1016/0378-1119(94)90025-6
Open Access Status Not Open Access
Volume 145
Issue 2
Start page 305
End page 310
Total pages 6
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
We report here the construction of vectors that produce and secrete human growth hormone (hGH) in a muscle-specific manner. The promoter regions of the genes encoding human skeletal α-actin (HSA) and troponin I slow (HTnIs) were linked to the hGH-encoding gene. These vectors were designated pHSA2000GH and pHTnIs4200GH, respectively. The HSA and HTnIs promoters linked to the cat gene have previously been shown to be necessary and sufficient for developmentally regulated muscle-specific expression. Furthermore, these promoters function in a fibre-type-specific manner in transgenic animals. Transient and stable transfection analyses with pHSA2000GH and pHTnIs4200GH indicated that: (i) these vectors efficiently synthesized hGH in a muscle-specific manner; (ii) the myogenic master regulatory gene, myoD, a determinant of cell fate, trans-activated expression of hGH in pluripotential non-muscle cells; and (iii) these hGH expression vectors were developmentally regulated during myogenic differentiation. These regulated tissue/fibre-type-specific hGH-containing plasmids are suitable vectors for the delivery and stable production of GH in livestock and GH-deficient hosts by either transgenesis, myoblast transfer or liposome-mediated intravenous injection.
Keyword Cloning
Transfection
Tissue-specific expression
Transgene
Gene therapy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Biomedical Sciences Publications
Institute for Molecular Bioscience - Publications
 
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