Expression of Two N1 Clones with Single Amino Acid Dissimilarity of Avian Influenza H5N1 Virus

Hartawan, Risza, Dharmayanti, Ni Luh Putu Indi, Robinson, Karl, Mahony, Timothy and Meers, Joanne (2012) Expression of Two N1 Clones with Single Amino Acid Dissimilarity of Avian Influenza H5N1 Virus. HAYATI Journal of Biosciences, 19 4: 169-176. doi:10.4308/hjb.19.4.169

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Author Hartawan, Risza
Dharmayanti, Ni Luh Putu Indi
Robinson, Karl
Mahony, Timothy
Meers, Joanne
Title Expression of Two N1 Clones with Single Amino Acid Dissimilarity of Avian Influenza H5N1 Virus
Journal name HAYATI Journal of Biosciences   Check publisher's open access policy
ISSN 1978-3019
2086-4094
Publication date 2012-11-19
Sub-type Article (original research)
DOI 10.4308/hjb.19.4.169
Open Access Status DOI
Volume 19
Issue 4
Start page 169
End page 176
Total pages 8
Place of publication Bogor, Indonesia
Publisher Indonesian Society for Biology
Language eng
Abstract Two clones of N1 gene derived from isolate A/Dk/Tangerang/Bbalitvet-ACIAR-TE11/2007 (H5N1) exhibit single mismatch of amino acid sequence at position 242 that is threonine and methionine for the clone #3 and #5, respectively. In order to evaluate the effect of the amino acid substitution, these clones were inserted into two different expression vectors that are pEGFP-C1 and pcDNA-3.3 TOPO® TA cloning. Subsequently, the respective recombinant clones were transfected into eukaryotic cells, including CEF, RK13 and VERO using Lipofectamine ‘plus’ reagent. As a result, the clone #3 retaining atypical sequence showed lower expression level rather than the clone #15 in both vectors and all type of cells. The 3D conformational modelling revealed that the mutation occurs in the inner part of glycoprotein embedded within envelope or matrix. Therefore, the missense mutation seems has no effect on the antigenic properties of neuraminidase but this substitution by any means causes lethal mutagenesis in the individual gene expression by reducing level of protein transcript.
Keyword Missense Mutation
In vitro expression
H5N1
N1 Gene
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes http://journal.ipb.ac.id/index.php/hayati/article/view/6533

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Created: Tue, 29 Jul 2014, 10:09:53 EST by Karl Robinson on behalf of Centre for Plant Science