Characterisation of the H5 and N1 genes of an Indonesian highly pathogenic avian influenza virus isolate by sequencing of multiple clone approach

Hartawan, Risza, Robinson, Karl, Mahony, Timothy and Meers, Joanne (2010) Characterisation of the H5 and N1 genes of an Indonesian highly pathogenic avian influenza virus isolate by sequencing of multiple clone approach. Indonesian Journal of Animal and Veterinary Sciences, 15 3: 240-251. doi:10.14334/jitv.v15i3.663


Author Hartawan, Risza
Robinson, Karl
Mahony, Timothy
Meers, Joanne
Title Characterisation of the H5 and N1 genes of an Indonesian highly pathogenic avian influenza virus isolate by sequencing of multiple clone approach
Journal name Indonesian Journal of Animal and Veterinary Sciences   Check publisher's open access policy
ISSN 0853-7380
Publication date 2010-03-04
Sub-type Article (original research)
DOI 10.14334/jitv.v15i3.663
Open Access Status DOI
Volume 15
Issue 3
Start page 240
End page 251
Total pages 12
Place of publication Bogor, Indonesia
Publisher Pusat Penelitian dan Pengembangan Peternakan / Center for Animal Science Research and Development
Language eng
Formatted abstract
Hemagglutinin and neuraminidase are the main antigenic determinants of highly pathogenic avian influenza (HPAI) virus. The features of these surface glycoproteins have been intensively studied at the molecular level. The objective of this research was to characterise the genes encoding these glycoproteins by sequencing of multiple clones. The H5 and N1 genes of isolate A/duck/Tangerang/Bbalitvet-ACIAR-TE11/2007 were each amplified in one or two fragments using reverse transcriptase-PCR (RT-PCR), and subsequently cloned into pGEM-T Easy TA cloning system. The sequencing result demonstrated high homology between respective clones but with several variations that were identified as single nucleotide polymorphisms (SNPs). A total of 1,707 base pair and 1,350 base pair of H5 and N1 genes respectively were successfully assembled from multiple clones containing the genes of interest. The features of both H5 and N1 genes from this isolate resemble the typical characteristics of Indonesian strains of H5N1 virus from sub-clade 2.1.3.
Keyword Avian influenza
Characterization
Gene Cloning
Hemagglutinin
neuraminidase
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes http://medpub.litbang.deptan.go.id/index.php/jitv/article/view/663

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
School of Veterinary Science Publications
 
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Created: Tue, 29 Jul 2014, 09:53:09 EST by Karl Robinson on behalf of Centre for Plant Science