Isoprene production in transgenic tobacco alters isoprenoid, non-structural carbohydrate and phenylpropanoid metabolism, and protects photosynthesis from drought stress

Tattini, Massimiliano, Velikova, Violeta, Vickers, Claudia, Brunetti, Cecilia, Di Ferdinando, Martina, Trivellini, Alice, Fineschi, Silvia, Agati, Giovanni, Ferrini, Francesco and Loreto, Francesco (2014) Isoprene production in transgenic tobacco alters isoprenoid, non-structural carbohydrate and phenylpropanoid metabolism, and protects photosynthesis from drought stress. Plant, Cell and Environment, 37 8: 1950-1964. doi:10.1111/pce.12350


Author Tattini, Massimiliano
Velikova, Violeta
Vickers, Claudia
Brunetti, Cecilia
Di Ferdinando, Martina
Trivellini, Alice
Fineschi, Silvia
Agati, Giovanni
Ferrini, Francesco
Loreto, Francesco
Title Isoprene production in transgenic tobacco alters isoprenoid, non-structural carbohydrate and phenylpropanoid metabolism, and protects photosynthesis from drought stress
Journal name Plant, Cell and Environment   Check publisher's open access policy
ISSN 1365-3040
0140-7791
Publication date 2014-08
Year available 2014
Sub-type Article (original research)
DOI 10.1111/pce.12350
Open Access Status
Volume 37
Issue 8
Start page 1950
End page 1964
Total pages 15
Place of publication West Sussex United Kingdom
Publisher Blackwell Publishing
Collection year 2015
Formatted abstract
Isoprene strengthens thylakoid membranes and scavenges stress-induced oxidative species. The idea that isoprene production might also influence isoprenoid and phenylpropanoid pathways under stress conditions was tested. We used transgenic tobacco to compare physiological and biochemical traits of isoprene-emitting (IE) and non-emitting (NE) plants exposed to severe drought and subsequent re-watering. Photosynthesis was less affected by drought in IE than in NE plants, and higher rates were also observed in IE than in NE plants recovering from drought. Isoprene emission was stimulated by mild drought. Under severe drought, isoprene emission declined, and levels of non-volatile isoprenoids, specifically de-epoxidated xanthophylls and abscisic acid (ABA), were higher in IE than in NE plants. Soluble sugars and phenylpropanoids were also higher in IE plants. After re-watering, IE plants maintained higher levels of metabolites, but isoprene emission was again higher than in unstressed plants. We suggest that isoprene production in transgenic tobacco triggered different responses, depending upon drought severity. Under drought, the observed trade-off between isoprene and non-volatile isoprenoids suggests that in IE plants isoprene acts as a short-term protectant, whereas non-volatile isoprenoids protect against severe, long-term damage. After drought, it is suggested that the capacity to emit isoprene might up-regulate production of non-volatile isoprenoids and phenylpropanoids, which may further protect IE leaves.
Keyword Abscisic acid (ABA)
Carotenoids
Flavonols
Hexoses signalling
MEP pathway
Xanthophylls
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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