The economics of virus-like particle and capsomere vaccines

Chuan, Yap P., Wibowo, Nani, Lua, Linda H. L. and Middelberg, Anton P. J. (2014) The economics of virus-like particle and capsomere vaccines. Biochemical Engineering Journal, 90 255-263. doi:10.1016/j.bej.2014.06.005

Author Chuan, Yap P.
Wibowo, Nani
Lua, Linda H. L.
Middelberg, Anton P. J.
Title The economics of virus-like particle and capsomere vaccines
Journal name Biochemical Engineering Journal   Check publisher's open access policy
ISSN 1873-295X
Publication date 2014-09-15
Sub-type Article (original research)
DOI 10.1016/j.bej.2014.06.005
Open Access Status
Volume 90
Start page 255
End page 263
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 2015
Language eng
Abstract Effective control of infectious diseases relies on new vaccine technologies that can quicken and broaden vaccine delivery. Novel modular virus-like particle (VLP) and capsomere technologies have been recently reported. These technologies utilize murine polyomavirus (MuPyV) VLPs and capsomeres as potent delivery systems to carry and display antigenic modules consisting of heterologous peptides, in the form of modular constructs capable of inducing high levels of specific antibodies against bacterial or viral antigens. These constructs are prepared using high-yield microbial synthesis, potentially enabling low-cost, rapid and scalable manufacture of new vaccines. To evaluate this potential, this study analyzes the economics of capsomere and VLP production using process simulation. Data here show that the unit production cost (UPC) for capsomere is up to 69% lower than that for VLP at the comparison scale (500. L fermentor), due to a simpler downstream process and a higher product yield. For VLP production, reactive diafiltration assembly was shown to have a UPC 30% lower than dilution assembly. Sensitivity analysis of uncertain process inputs with Monte Carlo simulations revealed a significant influence of final biomass concentration on UPC, contributing up to 50% of variance observed in the UPC probability distribution. Despite such process variability, optimized capsomere or VLP vaccine production, using a 500-L or 1500-L fermentor respectively, has more than 80% chance of producing vaccine at a cost less than 1 cent per dose based on a conservative assumption of 50. μg protein per vaccine dose. With a 10-kL fermentor, both the capsomere and VLP processes have productivity that could allow manufacture of 320 million vaccine doses in 2.3 and 4.7 days, respectively. This study confirms with quantitative data the possible economic, speed and scale benefits of the modular capsomere and VLP vaccine technologies, which can potentially redefine current vaccine distribution landscape and time-scale benchmarks.
Keyword Biosynthesis
Bioprocess design
Process integration
Microbial growth
Virus-like particleX
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 29 Jul 2014, 00:35:23 EST by System User on behalf of Aust Institute for Bioengineering & Nanotechnology