Antibody and T cell responses induced in chickens immunized with avian influenza virus N1 and NP DNA vaccine with chicken IL-15 and IL-18

Lim, Kian-Lam, Jazayeri, Seyed Davoud, Yeap, Swee Keong, Mohamed Alitheen, Noorjahan Banu, Bejo, Mohd Hair, Ideris, Aini and Omar, Abdul Rahman (2013) Antibody and T cell responses induced in chickens immunized with avian influenza virus N1 and NP DNA vaccine with chicken IL-15 and IL-18. Research in Veterinary Science, 95 3: 1224-1234. doi:10.1016/j.rvsc.2013.07.013


Author Lim, Kian-Lam
Jazayeri, Seyed Davoud
Yeap, Swee Keong
Mohamed Alitheen, Noorjahan Banu
Bejo, Mohd Hair
Ideris, Aini
Omar, Abdul Rahman
Title Antibody and T cell responses induced in chickens immunized with avian influenza virus N1 and NP DNA vaccine with chicken IL-15 and IL-18
Journal name Research in Veterinary Science   Check publisher's open access policy
ISSN 0034-5288
1532-2661
Publication date 2013
Sub-type Article (original research)
DOI 10.1016/j.rvsc.2013.07.013
Open Access Status
Volume 95
Issue 3
Start page 1224
End page 1234
Total pages 11
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Abstract We had examined the immunogenicity of a series of plasmid DNAs which include neuraminidase (NA) and nucleoprotein (NP) genes from avian influenza virus (AIV). The interleukin-15 (IL-15) and interleukin-18 (IL-18) as genetic adjuvants were used for immunization in combination with the N1 and NP AIV genes. In the first trial, 8 groups of chickens were established with 10 specific-pathogen-free (SPF) chickens per group while, in the second trial 7 SPF chickens per group were used. The overall N1 enzyme-linked immunosorbent assay (ELISA) titer in chickens immunized with the pDis/N1. +. pDis/IL-15 was higher compared to the chickens immunized with the pDis/N1 and this suggesting that chicken IL-15 could play a role in enhancing the humoral immune response. Besides that, the chickens that were immunized at 14-day-old (Trial 2) showed a higher N1 antibody titer compared to the chickens that were immunized at 1-day-old (Trial 1). Despite the delayed in NP antibody responses, the chickens co-administrated with IL-15 were able to induce earlier and higher antibody response compared to the pDis/NP and pDis/NP. +. pDis/IL-18 inoculated groups. The pDis/N1. +. pDis/IL-15 inoculated chickens also induced higher CD8+ T cells increase than the pDis/N1 group in both trials (P< 0.05). The flow cytometry results from both trials demonstrated that the pDis/N1. +. pDis/IL-18 groups were able to induce CD4+ T cells higher than the pDis/N1 group (P< 0.05). Meanwhile, pDis/N1. +. pDis/IL-18 group was able to induce CD8+ T cells higher than the pDis/N1 group (P<. 0.05) in Trial 2 only. In the present study, pDis/NP was not significant (P>. 0.05) in inducing CD4+ and CD8+ T cells when co-administered with the pDis/IL-18 in both trials in comparison to the pDis/NP. Our data suggest that the pDis/N1. +. pDis/IL-15 combination has the potential to be used as a DNA vaccine against AIV in chickens.
Keyword Avian influenza virus
CD4+ T cells
CD8+ T cells
DNA vaccine
IL-15
IL-18
N1
NP
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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