High resolution two-dimensional electrophoresis of native proteins

Weiland, Florian, Zammit, Carla M., Reith, Frank and Hoffmann, Peter (2014) High resolution two-dimensional electrophoresis of native proteins. Electrophoresis, 35 12-13: 1893-1902. doi:10.1002/elps.201400060

Author Weiland, Florian
Zammit, Carla M.
Reith, Frank
Hoffmann, Peter
Title High resolution two-dimensional electrophoresis of native proteins
Journal name Electrophoresis   Check publisher's open access policy
ISSN 1522-2683
Publication date 2014-07
Year available 2014
Sub-type Article (original research)
DOI 10.1002/elps.201400060
Open Access Status
Volume 35
Issue 12-13
Start page 1893
End page 1902
Total pages 10
Place of publication Weinheim, Germany
Publisher Wiley
Collection year 2015
Language eng
Formatted abstract
Blue native PAGE (BN-PAGE) is a powerful method to separate protein complexes while preserving their native state. However, the resolution of the method is limited as complexes with similar molecular masses cannot be resolved. Here we describe native 2DE using immobilized pH-gradients in combination with BN-PAGE to resolve protein complexes by their pI and molecular mass. This method enables electrophoretic separation of proteins between pI 3 and 10 and can resolve molecular masses up to 1.2 MDa. Visualized gel spots at large molecular weight were identified using MS to confirm potential protein complexes. Several protein complexes could be identified, most prominent GroEL in complex with GroES, parts of the ribosomal machinery and membrane transport system. In summary, this method enables easy high-resolution electrophoretic separation of protein complexes.
Keyword BN-PAGE
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Earth Sciences Publications
Official 2015 Collection
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Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
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